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Rt dulbecco s phosphate buffered saline dpbs 1x

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RT Dulbecco's Phosphate Buffered Saline (DPBS) (1x) is a sterile, isotonic buffer solution that maintains the pH and osmolarity of cell culture media. It is commonly used to wash, dilute, or resuspend cells in various biological applications.

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Lab products found in correlation

Recombinant mouse ifn γ, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Dulbecco's phosphate-buffered saline (DPBS) (1x) Dulbecco’s phosphate-buffered saline (DPBS) DPBS Dulbecco phosphate-buffered saline (DPBS, Dulbecco’s phosphate-buffered saline Phosphate buffered saline 1x Dulbecco’s phosphate DPBS 1X

2 protocols using rt dulbecco s phosphate buffered saline dpbs 1x

1

Infecting Macrophages with Leishmania Parasites

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As previously described (Sheel et al., 2015 (link)), mice were euthanized by CO2 asphyxiation and peritoneal lavage was performed using 5 mL of RT Dulbecco’s Phosphate Buffered Saline (DPBS) (1x) (GIBCO). Peritoneal cells were collected and washed with complete DMEM (10% (v/v) FCS containing 10 mmol L-glutamine, 100 mg/ml streptomycin and 100 U/ml penicillin), and 5 × 105 cells were seeded in 16-well glass chamber slides (Lab-Tek, Rochester, NY). Cells were then incubated at 37°C for 24 hours after which non-adherent cells were washed and removed with complete DMEM. LV9 amastigotes were added at a multiplicity of infection (MOI) of 10:1 (in 200 μL complete DMEM). After 1 hour at 37°C, free amastigotes were removed and cells were cultured for another 24 hours with 5 ng/ml recombinant mouse IFNγ (eBioscience, San Diego, CA). On the following day, cells were washed with 1x PBS, fixed and stained. Parasite infectivity was measured as number of parasites per 100 host macrophages.
Kumar R., Bunn P.T., Singh S.S., Ng S.S., de Oca M.M., De Labastida Rivera F., Chauhan S.B., Singh N., Faleiro R.J., Edwards C.L., Frame T.C., Sheel M., Austin R.J., Lane S.W., Bald T., Smyth M.J., Hill G.R., Best S.E., Haque A., Corvino D., Waddell N., Koufariotis L., Mukhopadhay P., Rai M., Chakravarty J., Singh O.P., Sacks D., Nylen S., Uzonna J., Sundar S, & Engwerda C.R. (2020). Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis. Cell reports, 30(8), 2512-2525.e9.
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2

Infecting Macrophages with Leishmania Parasites

Check if the same lab product or an alternative is used in the 5 most similar protocols
As previously described (Sheel et al., 2015 (link)), mice were euthanized by CO2 asphyxiation and peritoneal lavage was performed using 5 mL of RT Dulbecco’s Phosphate Buffered Saline (DPBS) (1x) (GIBCO). Peritoneal cells were collected and washed with complete DMEM (10% (v/v) FCS containing 10 mmol L-glutamine, 100 mg/ml streptomycin and 100 U/ml penicillin), and 5 × 105 cells were seeded in 16-well glass chamber slides (Lab-Tek, Rochester, NY). Cells were then incubated at 37°C for 24 hours after which non-adherent cells were washed and removed with complete DMEM. LV9 amastigotes were added at a multiplicity of infection (MOI) of 10:1 (in 200 μL complete DMEM). After 1 hour at 37°C, free amastigotes were removed and cells were cultured for another 24 hours with 5 ng/ml recombinant mouse IFNγ (eBioscience, San Diego, CA). On the following day, cells were washed with 1x PBS, fixed and stained. Parasite infectivity was measured as number of parasites per 100 host macrophages.
Kumar R., Bunn P.T., Singh S.S., Ng S.S., de Oca M.M., De Labastida Rivera F., Chauhan S.B., Singh N., Faleiro R.J., Edwards C.L., Frame T.C., Sheel M., Austin R.J., Lane S.W., Bald T., Smyth M.J., Hill G.R., Best S.E., Haque A., Corvino D., Waddell N., Koufariotis L., Mukhopadhay P., Rai M., Chakravarty J., Singh O.P., Sacks D., Nylen S., Uzonna J., Sundar S, & Engwerda C.R. (2020). Type I Interferons Suppress Anti-parasitic Immunity and Can Be Targeted to Improve Treatment of Visceral Leishmaniasis. Cell reports, 30(8), 2512-2525.e9.
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