Ain 93m diet and basal mix

Albumin from chicken egg white (OVA; grade V), cholera toxin (CT), collagenase, 1,4-dithiothreitol (DTT), ethylenediaminetetraacetic acid (EDTA), fetal bovine serum (FBS), Hanks balanced salt solution (HBSS), Roswell Park Memorial Institute (RPMI), Mayer’s hematoxylin solution, eosin Y solution, Percoll^®, Trizma^® base (Tris Base) and Tween 20 were purchased from Sigma-Aldrich (Madrid, Spain). Donkey serum was purchased from Jackson Immuno Research Laboratories (West Grove, PA, USA). NaN₃, p formaldehyde, tri-sodium citrate dihydrate and citric acid were provided by Merck (Darmstadt, Germany) and RNAlater^® by Ambion (Applied Biosystems, Austin, TX, USA). Xylene and 4',6-diamidino-2-phenylindole (DAPI) was purchased from Thermo Fisher Scientific (Vienna, Austria) and ethanol absolute from VWR (Vienna, Austria). Fluoromont G was provided by SouthernBiotech (Birmingham, AL, USA). Ketamine was obtained from Merial Laboratories S.A. (Barcelona, Spain) and xylazine from Bayer A.G. (Leverkusen, Germany). Natural Forastero cocoa was obtained from Idilia Foods SL (formerly Nutrexpa S.L., Barcelona, Spain) and AIN-93M diet and basal mix from Harlan Teklad (Madison, Wisconsin, USA).