The RSC96 cell line was established via spontaneous transformation of long-cultured primary cultures of rat Schwann cells. RSC96 cells (ATCC) and HEK293T cells (Type Culture Collection of the Chinese Academy of Medical Sciences) were cultured in DMEM containing 10% fetal bovine serum (FBS, Gibco, Waltham, MA, USA), while a normal human thyroid follicular epithelial cell line (Nthy-ori 3-1 cells, Stem Cell Bank/Stem Cell Core Facility) was cultured in RPMI-1640 medium (Hyclone, South Logan, UT, USA) supplemented with 10% FBS. Furthermore, the medium used for producing lentivirus using HEK293T cells was DMEM supplemented with 30% FBS. All the cells were cultured at 37°C in 5% CO2. After a 12 h incubation in a serum-free medium, the cells were treated with or without HG and PA and with or without TSH (ProSpec-Tany TechnoGene Ltd., Rehovot, Israel). PA was solubilized in 0.1 M NaOH and mixed with 20% BSA. Mannitol was used to match the hyperosmolality of the cells cultured under hyperglycemic conditions.
Rsc96 cells
Manufactured by Thermo Fisher Scientific
Sourced in United States
The RSC96 cells are a specialized laboratory equipment designed for high-throughput sample processing. They provide a consistent and efficient platform for various applications, such as automated liquid handling, sample preparation, and assay processing. The core function of the RSC96 cells is to facilitate the accurate and reproducible handling of a large number of samples simultaneously, enabling researchers to streamline their workflow and increase productivity.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Hek293t cells, by Thermo Fisher Scientific (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
High glucose dmem, by Thermo Fisher Scientific (1 mentions)
Dmem high glucose, by Merck Group (1 mentions)
Streptomycin, by Merck Group (1 mentions)
Penicillin, by Merck Group (1 mentions)
Calf serum, by Thermo Fisher Scientific (1 mentions)
C2c12 cells, by Merck Group (1 mentions)
Sb216763, by Apexbio (1 mentions)
2 protocols using rsc96 cells
1
Rat Schwann Cell Transformation Protocol
2
Murine Schwann and Myoblast Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Murine RSC96 Schwann cell and C2C12 myoblast cell lines, passages 1–3, were obtained from American Type Culture Collection (Manassas, VA, USA). Cells were cultured in growth media: RSC96 cells with Dulbecco's modified Eagle's medium (DMEM)/F12 containing 10% fetal bovine serum (Gibco, Grand Island, NY, USA), 100 units/mL penicillin and 100 µg/mL streptomycin (Sigma-Aldrich, St. Louis, MO, USA); C2C12 cells with high-glucose DMEM (Sigma-Aldrich) containing 5 mM glucose, 10% fetal bovine serum and antibiotics., When cells reached 80–90% confluence, the medium was replaced by a differentiation medium (high-glucose DMEM containing 2% calf serum (Gibco) and antibiotics) to induce C2C12 cell myotube formation and differentiation. The differentiation medium was changed daily for 6 days. SB216763 (Apexbio, Houston, TX, USA) was prepared as a 20 mM stock in dimethyl sulfoxide. RSC96 and C2C12 cells were exposed to 10 µM of SB216763 for 48 hours as described previously (Chen et al., 2016). Following these treatments, cells were washed with phosphate-buffered saline (PBS; Gibco) to remove any free drug prior to the sequence experiments.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!