Cefprozil monohydrate

CFPAC-1 cells were seeded on glass coverslips in 12-well plates containing 1 ml of culture medium per well. At 30% confluency, cell media was replaced by low serum medium containing 2% FBS. After 8 days of compound treatment, cells were fixed with 4% Formaldehyde (Merck), permeabilized with 0.1% Triton X100 (Merck) in DPBS, blocked with 1% BSA (Applichem) in DPBS, and incubated with 1:1000 dilution of anti-acetylated tubulin antibody or 1:500 dilution of anti-IFT88 antibody (Cat. No. 13967–1-AP, Proteintech) for 1 h, followed by incubation with 1:1000 dilution of fluorescent secondary antibody for 1 h. Nuclei were counterstained with DAPI (Vector Laboratories, Vectashield (Cat. No. H-1500). Images of primary cilia were captured by acquiring Z-stacks using either a Bio-Rad Radiance or Nikon C2 Eclipse Ti-E confocal laser scanning microscope by 40X or 60X oil immersion lenses. All drugs selected for confocal reconfirmation experiments were purchased from Sigma, except for Almotriptan Malate and Sirolimus which were obtained from Selleckchem. Gefitinib was from Invivogen, Cefprozil Monohydrate from Abcam and Imexon from MicroSource Discovery Systems Inc.

Esomeprazole, Pargyline, Leflunomide, Clofibrate, Ciprofloxacin, Ketorolac, Paroxetine, Dobutamine, Tranilast, Loperamide, Xylazine, Capsaicin, Papaverine, Oxymetazoline, Dexamethasone, Docetaxel, Topotecan, Paclitaxel were purchased from Sigma. Almotriptan Malate and Sirolimus were purchased from Selleckchem, Gefitinib from Invivogen, Cefprozil Monohydrate from Abcam and Imexon from MicroSource Discovery Systems Inc.; ATP, Apyrase, Suramin and Mefloquine were purchased from Sigma; Latrunculin B was purchased from Abcam.