Ab137855

The different antibodies used were mouse monoclonal anti-K8 antibody (clone M20, Sigma-Aldrich, Darmstadt, Germany), mouse monoclonal anti-C terminal K8 antibody (clone 1E8, Abcam, Cambridge, UK), rabbit polyclonal anti-C terminal K8 antibody (SAB4501654, Sigma-Aldrich, Darmstadt, Germany), rabbit polyclonal anti-N terminal K8 antibody (ab137855, Abcam, Cambridge, UK), proprietary mouse monoclonal anti-K8 antibodies (clone D-A10 and clone D-D6), mouse monoclonal anti-uPA antibody (ab131433, Abcam, Cambridge, UK), mouse monoclonal anti-plasminogen antibody (ab38157 Abcam, Cambridge, UK), rabbit polyclonal anti-caspase 3 cleaved (Asp 175) antibody for IF analysis (9661, Cell Signaling Technology, Leiden, The Netherlands), rabbit monoclonal anti-cleaved caspase 3 antibody for IHC analysis (clone 5A1E, Cell Signaling Technology, Leiden, The Netherlands), mouse monoclonal anti-caspase 3 and cleaved caspase 3 Alexis antibody for Western blot (WB) analysis (Enzo Life Science, Farmingdale, NY, USA), mouse monoclonal anti-Ku80 antibody (ab3715, Abcam, Cambridge, UK), mouse monoclonal anti-histone H3 antibody (ab1791, Abcam, Cambridge, UK), anti-Na+/K+ ATPase alpha (H3) (SC-48345, Santa Cruz Biotechnology, Santa Cruz, CA, USA) rabbit polyclonal anti-GFP antibody (ab290, Abcam, Cambridge, UK), and mouse monoclonal anti-Ki67 antibody (Clone Mib1, M7240 DAKO, Santa Clara, CA, USA).

To investigate cell viability and apoptosis, cells cultured on the scaffold were observed by double staining with fluorescein diacetate (FDA) and propidium iodide (PI). A scanning electron microscope (SEM, JSM-6380LV, JEOL, Tokyo, Japan) was used to visualize the surface features of the scaffold and cells cultured in the scaffold. The samples were visualized with the SEM using an accelerating voltage of 30 kv. For detailed information on SEM sample preparation, see S1 Text. For Immunocytochemistry, the cultures were prepared as previously described [27 (link)]. The primary antibodies used were as follows: polyclonal rabbit anti-cytokeratin 8 antibody (CK8, 1:100, ab137855, Abcam) and monoclonal rat anti-CD44 antibody (1:100, ab119348, Abcam). Then, followed by secondary goat anti-mouse IgG-TRITC (1:1000, ab6786, Abcam), or a goat anti-rabbit IgG-FITC (1:1000, ab7086, Abcam) antibody.