3.5 μl of purified particles were deposited onto glow-discharged, 300 Mesh Copper carbon-coated grids (Quantifoil, Germany). After a 1 min incubation at room temperature, excess liquid was blotted and the grids were stained with two successive drops of 1% uranyl acetate (3.5 μl each). The first drop was immediately blotted, the second was left for ∼20 s on the grids before blotting. Finally, the grids were air-dried before observation on a Jeol 2100 microscope of the METI platform (Centre de Biologie Intégrative, Toulouse, France), equipped with a LaB6 cathode and operating at 200 kV. Images were recorded with defocus ranging from 0.6 to 2.0 μm, on a Gatan US1000 CCD digital camera and DigitalMicrograph (Gatan, Inc.), at various magnifications corresponding to calibrated pixel sizes of 3.4, 1.7 and 1.1 Å.
Us1000 ccd digital camera
Manufactured by Ametek
Sourced in France
The US1000 CCD digital camera is a laboratory instrument designed for scientific and industrial applications. It features a high-resolution charge-coupled device (CCD) sensor that captures digital images with precision. The camera's core function is to provide accurate and reliable image capture for various analytical and research purposes.
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Lab products found in correlation
2 protocols using us1000 ccd digital camera
1
Transmission Electron Microscopy of Purified Particles
2
High-Resolution Imaging of Mucin-GNP Complex
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High-resolution transmission electron micrographs were obtained with a JEOL 3010-UHR instrument operating at 300 kV, equipped with a 2k × 2k pixels Gatan US1000 CCD digital camera.
Samples of purified mucin-GNPs complex were dried on a carbon-coated copper grid. The nominal magnification used to record the complex mucin-GNPS were x500000 and x800000.
Samples of purified mucin-GNPs complex were dried on a carbon-coated copper grid. The nominal magnification used to record the complex mucin-GNPS were x500000 and x800000.
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