WT or BM8 mice were irradiated with 900 Rads and rested for 4 hours before reconstitution with either WT, BM8, or CD11c-DTR bone marrow. Bone marrow was isolated and red blood cells were lysed prior to intravenous transfer. At 12 weeks post reconstitution, mice were immunized as described above. For CD11c DTR experiments, mice were vaccinated and 100ng of diptheria toxin (DT) (Sigma Aldrich) was injected intraperitoneally at the indicated time points as previously described 23 (link).
Diptheria toxin
Manufactured by Merck Group
Diphtheria Toxin is a laboratory reagent used in scientific research. It is a purified protein derived from the bacterium Corynebacterium diphtheriae, the causative agent of diphtheria. The core function of Diphtheria Toxin is to serve as a tool for investigating cellular processes and protein interactions, particularly in the context of diphtheria and related diseases.
Automatically generated - may contain errors
Lab products found in correlation
Pen strep, by Thermo Fisher Scientific (1 mentions)
Mem non essential amino acid, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hygromycin, by Thermo Fisher Scientific (1 mentions)
β mercaptoethanol, by Thermo Fisher Scientific (1 mentions)
Rpmi 1640 plus l glutamine, by Thermo Fisher Scientific (1 mentions)
5 protocols using diptheria toxin
1
Investigating CD11c+ Cell Function in Immunization
2
Investigating CD11c+ Cell Function in Immunization
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WT or BM8 mice were irradiated with 900 Rads and rested for 4 hours before reconstitution with either WT, BM8, or CD11c-DTR bone marrow. Bone marrow was isolated and red blood cells were lysed prior to intravenous transfer. At 12 weeks post reconstitution, mice were immunized as described above. For CD11c DTR experiments, mice were vaccinated and 100ng of diptheria toxin (DT) (Sigma Aldrich) was injected intraperitoneally at the indicated time points as previously described 23 (link).
3
Diptheria Toxin Induced Immunosuppression
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Mice were intraperitonially (i.p.) injected with 200 ng of Diptheria Toxin (Sigma Aldrich) twice daily for 5 consecutive days. Mice were maintained on a sulfatrim diet and 0.5 mg/ml oral fluconazole (Citron Pharma) supplemented in drinking water throughout the course of treatment and subsequent wounding experiment.
4
Diptheria Toxin Induced Immunosuppression
5
Cell Culture Protocols for Immune Cell Studies
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‘Complete medium’ consisted of RPMI-1640 plus L-glutamine (Gibco) supplemented with 10% FBS (Brazilian source, ThermoFisher), 50μM β-Mercaptoethanol (Gibco), and PenStrep (Gibco) at 100U/mL penicillin and 100μg/mL streptomycin. Renca cells (RRID:CVCL_2174) were maintained in complete medium. CL4 and 5C.C7 T cells were maintained in complete medium supplemented with 50U/mL rh-IL-2 (NIH/NCI BRB preclinical repository)(‘IL-2 medium’). HEK293T (RRID:CVCL_0063) and Phoenix-E cells (RRID:CVCL_H717) were maintained in ‘Phoenix incomplete medium’ consisting of DMEM with 4.5g/L D-glucose, L-glutamine and sodium pyruvate (Gibco) supplemented with 10% FBS (Brazilian source, ThermoFisher), MEM non-essential amino acids (Gibco), and PenStrep (Gibco) at 100U/mL penicillin and 100μg/mL streptomycin. Long-term Phoenix cell stocks were kept in ‘Phoenix complete medium’, which is Phoenix incomplete medium supplemented with 300μg/mL Hygromycin (Invitrogen) and 1μg/mL Diptheria toxin (Sigma).
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