We cultured GECs and podocytes on six-well plates or chamber slides (Thermo Scientific, Waltham, MA, USA). We incubated cells with PKH26-labelled EVs for 1 h, and then cells seeded on chamber slides were fixed with paraformaldehyde (Sigma Aldrich), nuclei were counterstained in blue by 2.5 μg/mL Hoechst (Sigma Aldrich), evaluated by confocal microscopy (Zeiss LSM 5 PASCAL, Jena, Germany). Cells cultured on six-well plates were detached by EDTA (Sigma) and analyzed by FACS (FACS Calibur, Becton Dickinson, Franklin Lakes, NJ, USA). In selected experiments, PKH26-labelled EVs were pre-incubated with 1 μg/mL of different antibodies directed to block the binding to αVβ-3 integrin (Biolegend, San Diego, CA, USA), α4-integrin, α6-integrin (Chemicon, Temecula, CA), CD29 or L-selectin (Becton Dickinson).
α4 integrin
Manufactured by Merck Group
Sourced in United States
α4-integrin is a cell surface receptor protein that mediates cell-cell and cell-extracellular matrix interactions. It is involved in the regulation of various cellular processes, including adhesion, migration, and signaling.
Automatically generated - may contain errors
Lab products found in correlation
αvβ3 integrin, by BioLegend (2 mentions)
L selectin, by BD (2 mentions)
Lsm 5 pascal, by Zeiss (2 mentions)
Facscalibur, by BD (2 mentions)
Integrin α6, by Merck Group (2 mentions)
Paraformaldehyde (pfa), by Merck Group (2 mentions)
Ethylene diamine tetraacetic acid (edta), by Merck Group (2 mentions)
Hoechst, by Merck Group (2 mentions)
Chamber slide, by Thermo Fisher Scientific (2 mentions)
2 protocols using α4 integrin
1
Tracking EV Uptake in GECs and Podocytes
2
Internalization of EVs by GECs and Podocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
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We cultured GECs and podocytes on 6-well plates or chamber slides (Thermo Scientific, Waltham, MA, USA). We incubated cells with PKH26-labelled EVs for 1 h on cells seeded on chamber slides were fixed with paraformaldehyde (Sigma Aldrich), nuclei were counterstained in blue by 2.5 μg/mL Hoechst (Sigma Aldrich), evaluated by confocal microscopy (Zeiss LSM 5 PASCAL, Jena, Germany). Cells cultured on 6-well plates were detached by EDTA (Sigma) and analyzed by FACS (FACS Calibur, Becton Dickinson, Franklin Lakes, NJ, USA). In selected experiments, PKH26-labelled EVs were pre-incubated with 1 μg/mL of different antibodies directed to block the binding to αVβ3integrin (Biolegend, San Diego, CA, USA), α4-integrin, α6-integrin (Chemicon, Temecula, CA), CD29 or L-selectin (Becton Dickinson).
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