L 012 sodium salt

Luminol (sodium salt) was purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). L-012 sodium salt was purchased from Wako Chemicals USA (Wako Chemicals USA, Inc., Richmond, VA, USA). D-luciferin, potassium salt was purchased from Gold Biotechnology, Inc.^® (Gold Biotechnology, Inc.^®, St. Louis, MO, USA). TNFα was purchased from R&D Systems, Inc. (R&D Systems, Inc., Minneapolis, MN, USA). Luminol sodium salt was dissolved in sterile phosphate-buffered saline (PBS) to a final concentration of 50 mg/mL and stored at −20 °C. Phorbol 12-myristate 13-acetate (PMA) was freshly prepared from a 20 mM stock solution (dissolved in DMSO, stored at −20 °C) and diluted in PBS to a final concentration of 2 mM PMA. L-012 powder was dissolved in sterile double distilled water (ddH₂0) to a final concentration of 20 mM and stored at −20 °C. Puromycin was purchased from Thermo Fisher Scientific (Thermo Fisher Scientific Inc., Waltham, MA, USA).

Luminol (sodium salt), and lucigenin (N,N′-Dimethyl-9,9′-biacridinium dinitrate) were purchased from Sigma-Aldrich (Sigma-Aldrich, MO, USA). L-012 sodium salt was purchased from Wako Chemicals USA (Wako Chemicals USA, Inc., Richmond, VA, USA). Verdiperstat, AZD5904 and hydroxypropyl-β-cyclodextrin (HP-β-CD) were purchased from MedChemExpress (MedChemExpress LLC, NJ, USA). Anti-CTLA-4 (9D9), anti-αPD-1 (RMP1-14), anti-Ly6G (1A8) and polyclonal rat IgG control were obtained from Bio X cell (Bio X cell, NH, USA). Luminol sodium salt was dissolved in sterile phosphate-buffered saline (PBS) to a final concentration of 50 mg/mL or 100 mg/mL and stored at − 20ºC. L-012 powder was dissolved in sterile double distilled water (ddH₂0) to a final concentration of 20 mM and stored at − 20ºC. lucigenin was dissolved in sterile PBS to a final concentration of 1 mg/mL and made fresh for each imaging session. Verdiperstat and AZD5904 were dissolved in sterile DMSO to a final concentration of 10 mM and stored at − 20ºC; AZD5904 – 5% DMSO, 15% HP-β-CD w/v, 200µL intraperitoneal injection daily, Verdiperstat – 4% DMSO,15% HP-β-CD w/v, 300µL intraperitoneal injection daily.