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Pe rat anti mouse cd31 antibody

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The PE rat anti-mouse CD31 antibody is a laboratory reagent used to detect the presence of the CD31 protein, also known as platelet endothelial cell adhesion molecule (PECAM-1), in mouse samples. CD31 is a cell surface marker expressed on endothelial cells and is commonly used to identify and study these cells in various research applications.

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Lab products found in correlation

Dab substrate kit for peroxidase, by Vector Laboratories (1 mentions) Anti mouse cd16 32 antibody, by Thermo Fisher Scientific (1 mentions) Anti sapk jnk antibody, by Cell Signaling Technology (1 mentions) Anti neutrophil antibody 7 4, by Abcam (1 mentions) Anti phospho jnk, by Santa Cruz Biotechnology (1 mentions) Streptavidin pe, by Thermo Fisher Scientific (1 mentions) Streptavidin fitc, by Thermo Fisher Scientific (1 mentions) Streptavidin hrp, by BioLegend (1 mentions) Lps escherichia coli 0111 b4, by Merck Group (1 mentions) Percp cy5.5 anti mouse cd45 antibody, by BD (1 mentions) Facsaria 3, by BD (1 mentions) Dispase 2, by Roche (1 mentions) Collagenase 1, by Roche (1 mentions) Vibratome, by Leica (1 mentions) Sp5 confocal microscope, by Leica (1 mentions)

Spelling variants of this product

Rat anti-mouse CD31 (109 citations) Rat anti-CD31 (90 citations) Rat anti-mouse CD31 antibody (65 citations) Anti-CD31 antibody (62 citations) Anti-mouse CD31 antibody (27 citations) Anti-mouse CD31 (27 citations) Anti-CD31-PE (25 citations) Rat anti-CD31 antibody (20 citations) PE rat anti-mouse CD31 (9 citations) Anti-CD31-PE antibody (2 citations)

5 protocols using pe rat anti mouse cd31 antibody

1

In Vivo Glioma Imaging with Q-NPs

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Tumor-bearing mice were established through the intracranial injection of GL261 cells that were engineered to express GFP. After 21 days, the mice were treated with Cy5-labeled Q-NPs. At 12- and 24-h time points, a cohort of mice was intravenously administered 100 μL PE rat anti-mouse CD31 antibody (BD PharMingen #553373). One hour later, the mice were euthanized. The brains were isolated, sliced, subjected to DAPI staining, and imaged by confocal microscopy.
Liu F., Peng B., Li M., Ma J., Deng G., Zhang S., Sheu W.C., Zou P., Wu H., Liu J., Chen A.T., Mohammed F.S, & Zhou J. (2021). Targeted disruption of tumor vasculature via polyphenol nanoparticles to improve brain cancer treatment. Cell reports. Physical science, 3(1), 100691.
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2

Macrophage Activation and Inflammation Regulation

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LPS (Escherichia coli 0111:B4) was purchased from Sigma (St. Louis, MO). TUDCA was purchased from Prodotti Chimici E Alimentari S.p.A (Basaluzzo, Italy). Murine macrophage colony-stimulating factor (M-CSF), CCL3 and CCL5 were obtained from PeproTech (Rocky Hill, NJ). Anti-mouse monocyte/macrophage marker (MOMA-2) antibody and anti-phospho-JNK were purchased from Santa Cruz (Dallas, TX); anti-mouse CD 16/32 antibody, anti-mouse Ly-6G (Gr-1) antibody, biotin-anti-mouse IgG, biotin-anti-rat IgG and streptavidin-PE, streptavidin-FITC were from eBioscience (San Diego, CA). PE-rat anti-mouse CD31 antibody and biotin-goat anti-rabbit Ig antibody were from BD Pharmingen (San Jose, CA). PE-anti-mouse TGF-β antibody and streptavidin-HRP were from Biolegend (San Diego, CA). Anti-neutrophil antibody (7/4) was from Abcam (Cambridge, MA). Dab substrate kit for peroxidase was from Vector Laboratories (Burlingame, CA). Anti-SAPK/JNK antibody was obtained from Cell Signaling Technology.
Yuan R., Geng S., Chen K., Diao N., Chu H.W, & Li L. (2016). Low-grade inflammatory polarization of monocytes impairs wound healing. The Journal of pathology, 238(4), 571-583.
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3

Isolation of Lung Endothelial Cells

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To obtain lung endothelial cells, the lung tissues were digested with Collagenase I (Roche, 11088793001) plus Dispase II (Roche, 04942078001) at a working concentration of 2 mg/mL for 30 min at 37 °C. Red blood cells were then disintegrated using red blood lysis buffer (155 mM NH4Cl,12 mM NaHCO3, 0.1 mM EDTA). The remaining viable cells were incubated with PerCP-Cy™5.5 Anti-Mouse CD45 antibody (BD, 550994) and PE Rat Anti-Mouse CD31 antibody (BD, 553373) at 4 °C under gentle rotation for 30 min. After centrifugation, the cells were washed once with PBS containing 1% donkey serum and 2 mM EDTA. Finally, flow cytometry sorting was performed to obtain CD45−CD31+ lung endothelial cells with FACS Aria Ⅲ (BD Biosciences).
Chen X., Wang H., Wu C., Li X., Huang X., Ren Y., Pu Q., Cao Z., Tang X, & Ding B.S. (2024). Endothelial H2S-AMPK dysfunction upregulates the angiocrine factor PAI-1 and contributes to lung fibrosis. Redox Biology, 70, 103038.
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4

In Vivo Glioma Imaging with Q-NPs

Check if the same lab product or an alternative is used in the 5 most similar protocols
Tumor-bearing mice were established through the intracranial injection of GL261 cells that were engineered to express GFP. After 21 days, the mice were treated with Cy5-labeled Q-NPs. At 12- and 24-h time points, a cohort of mice was intravenously administered 100 μL PE rat anti-mouse CD31 antibody (BD PharMingen #553373). One hour later, the mice were euthanized. The brains were isolated, sliced, subjected to DAPI staining, and imaged by confocal microscopy.
Liu F., Peng B., Li M., Ma J., Deng G., Zhang S., Sheu W.C., Zou P., Wu H., Liu J., Chen A.T., Mohammed F.S, & Zhou J. (2021). Targeted disruption of tumor vasculature via polyphenol nanoparticles to improve brain cancer treatment. Cell reports. Physical science, 3(1), 100691.
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5

Visualizing Glioma Vasculature in Mice

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Mice containing gliomas received four ABTT NP injections. Two days after NP injections, 100 μL of PE Rat Anti-Mouse CD31 antibody (BD Pharmingen # 553373) was injected intravenously to label the tumor vasculature. One hour after injecting the antibody, mice were perfused with 1x PBS followed by 4% paraformaldehyde (PFA). Brains were incubated overnight in 4 % PFA and 60 μm thick sections were obtained using a vibratome (Leica). Tumor-containing brain sections were mounted and used for high-resolution confocal imaging. Leica SP5 confocal microscope with 10× air, 40× and 63× objectives with APO oil immersion were used to obtain Z-stacks at 0.5 μm step sizes and zooms from 1 to 5. Images were processed using NIH ImageJ.
Han L., Kong D.K., Zheng M.Q., Murikinati S., Ma C., Yuan P., Li L., Tian D., Cai Q., Ye C., Holden D., Park J.H., Gao X., Thomas J.L., Grutzendler J., Carson R.E., Huang Y., Piepmeier J.M, & Zhou J. (2016). Increased Nanoparticle Delivery to Brain Tumors by Autocatalytic Priming for Improved Treatment and Imaging. ACS nano, 10(4), 4209-4218.
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