Ultra-high-resolution spectral domain optical coherence tomography (OCT) system (Envisu R2210 UHR Leica Microsystems Inc.) was used for in vivo cross-sectional imaging. The scan parameters used were 1.8 × 1.8 mm rectangular volume scan, 1000 a-scans/200 b-scans averaged three times per b-scan. The B-scan was used to measure the width (a) and depth (b) of the lesion. The volume intensity projection (VIP) image was used to measure the length (c) of the lesion. The lesion volume was calculated using the formula A = 43πabc. Immediately after the laser injury, each animal was scanned for ruptured Bruch’s membrane to confirm successful CNV induction. The inclusion criteria to include a lesion in the quantitative analysis is as described62 (link). Each CNV lesion was then followed over time using the same scan parameters at day 7, 14, and 21. The volume of each lesion was calculated with OCT volumetric scans using previously described methods62 (link).
Envisu r2210
Manufactured by Leica
Sourced in Germany
The Envisu R2210 is a high-resolution spectral-domain optical coherence tomography (SD-OCT) system designed for preclinical research. It provides micrometer-scale, depth-resolved imaging of biological samples.
Automatically generated - may contain errors
6 protocols using envisu r2210
1
In Vivo Quantification of Choroidal Neovascularization
2
Pupil Dilation and Ocular Imaging in Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In brief, mice were anesthetized, and pupils were dilated with 5 mg/mL tropicamide (Mydrum; Bausch + Lomb, Rochester, NY, USA) and phenylephrine (Neosynephrin-POS 10%; Ursapharm, New Delhi, India). Optical coherence tomography (OCT) recordings were done with a Bioptigen Envisu R2210 device (Leica Microsystems, Wetzlar, Germany) equipped with a lens designed for mouse eyes (50° field of view). Fundus pictures were recorded with a Micron IV Fundus camera (Phoenix Research Laboratories, Pleasanton, CA, USA). Pictures of mouse eyes (pupil size) were taken with a Spectralis HRA/OCT device (Heidelberg Engineering, Heidelberg, Germany) approximately 20 minutes after pupil dilation as described above. The same eyes were imaged three or four times: Before AAV injection (pretreatment value) and one week (AAV-stuffer control only) and three and six weeks after AAV injection.
3
Corneal Imaging for UVB Damage
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Slit lamp and OCT image detections were applied at 7 d post-UVB irradiation. Slit lamp detection was performed in a dark environment. Rats were anesthetized as previously described, and digital slit lamp (LS-5, Chongqing Shangbang Medical Equipment Co., Ltd.) detection was applied using direct and indirect methods as the operator manuals. The area of cornea desquamation and neovascularization was identified by Image-Pro Plus 6.0 software. The OCT detection procedure complied with the operator manual (Leica Bioptigen Envisu R2210, Leica Microsystems, Germany) using a 10 mm telecentric lens. The thickness of the cornea was calculated with an OCT Image Analysis Software.
4
Corneal Structure Imaging and Scar Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Before and immediately after injury, and at time interval, cross-sectional corneal structure were scanned (4 mm diameter) with Spectral Domain Ophthalmic Coherence Tomography (SD-OCT, Envisu R2210, Leica [Bioptigen], Morrisville, NC). Images were processed with NIS-Elements software (Nikon, Melville, NY) in a masked fashion. Central corneal thickness (CCT) was measured as the mean of 3 measurements obtained at the centre (0 mm) and at 0.5 mm on either side [31] (link). At day 10 post-treatment, mice were sacrificed with overdose of sodium pentobarbital, followed by cervical dislocation. All corneas were isolated under dissecting microscopy, imaged and the scar area was determined in a masked fashion using the Fiji open-source image analysis software package (https://fiji.sc/ ) and MetaMorph 7.7.3 (Molecular Devices Inc., San Jose, CA) [10] . Threshold images were generated after digitally removing the corneal epithelium. Naïve corneas were used to set the threshold value for scar volume measurement. Statistical analyses were performed with Prism 7 (GraphPad Prism) using paired Student’s t-test.
5
Noninvasive Imaging of Ocular Vasculature
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A fixed pressure, gravity-based infusion was chosen, with IOP set by a water column height of 30 cm, equivalent to 22 mmHg. The IOP had to be kept as stable as possible to avoid changes due to fluctuating vascular inflation and to prevent hypotony with unstable mounts, which can occur after AIT. All eyes were allowed to equilibrate with the control perfusate for 1 h. We used SD-OCT imaging (Envisu R2210, Leica, Morrisville, NC, USA) for repeatable, noninvasive, high-resolution assessment of tissue morphology. Scan cubes were 6.0 mm along the limbus, 4.0 mm wide, and 1.6 mm deep and were acquired every 20 min for a total of 3 h. The imaging head was tilted to keep the 10-mm telecentric lens perpendicular and parallel to the limbus. All images were processed using image processing software (ImageJ 1.50b, http://imagej.nih.gov/ij , Wayne Rasband, NIH) to remove some of the background noise, extract signal voids from the stacks, and convert to TIFF files. All 10 data cubes per eye were then imported into Avizo 3D software (v2021.2, FEI; ThermoScientific) for denoising, 3D reconstruction, and realignment of the imaged structures. Areas with vessels were automatically detected, and their volumes were measured. For each eye, the volumes at all ten time points were used for linear regression. This regression was used to determine the relative volume change.
6
Assessing Corneal Scarring Post-Injury
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Before and after injury, as well as at weekly intervals, a cross-sectional corneal scanning (4 × 4 mm area) was performed using a Spectral Domain Optical Coherence Tomography (SD-OCT, Envisu R2210, Leica [Bioptigen], Morrisville, NC). The obtained images were processed with NIS-Elements software (Nikon, Melville, NY) in a masked fashion. Corneal thickness was measured by taking the average of three measurements obtained at the center (0 mm) and at 0.5 mm on both sides [18 (link)]. On day 10, the mice was euthanized by administering an overdose of intraperitoneal sodium pentobarbital injection, followed by cervical dislocation. All isolated corneas were imaged, and the scar area was determined using Fiji, an open-source image analysis software package (https://fiji.sc/ ), in a blinded manner [8 (link)].
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!