Econo column

Manufactured by Merck Group

The Econo-Column is a compact, versatile chromatography column designed for a variety of laboratory applications. It features a simple, user-friendly design and is suitable for purification, concentration, or desalting of various biomolecules.

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Lab products found in correlation

Deae sepharose fast flow, by Merck Group (2 mentions) Pd 10 sephadex g 25m, by Merck Group (2 mentions) Spectra chrom lc, by Merck Group (2 mentions) Amicon stirred cell concentrators, by Merck Group (2 mentions) Ultrafiltration membranes 10 000 da mw cutoff, by Merck Group (1 mentions) Ultrafiltration membrane, by Merck Group (1 mentions)

2 protocols using econo column

Synthesis and Purification of 2-Oxo-3-pentynoate

Cited 1 time

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All chemicals, biochemical, and enzyme substrates were obtained from sources described elsewhere.^{19 (link),20} 2-Oxo-3-pentynoate (2-OP) was synthesized as reported.^{21 (link)} Chromatographic resins (DEAE Sepharose fast flow and Bio-Gel P60 gel beads) and columns (PD-10 Sephadex G-25M, Econo-Column, and Spectra/Chrom LC) were acquired from sources described elsewhere.^{19 (link)} The Amicon stirred cell concentrators and the ultrafiltration membranes (10,000 Da, MW cutoff) were purchased from EMD Millipore Inc. (Billerica, MA). InstantBlue for staining SDS-PAGE gels was obtained from C.B.S. Scientific Company, Inc. (Del Mar, CA).

Baas B.J., Medellin B.P., LeVieux J.A., de Ruijter M., Zhang Y.J., Brown S.D., Akiva E., Babbitt P.C, & Whitman C.P. (2019). Structural, Kinetic, and Mechanistic Analysis of an Asymmetric 4-Oxalocrotonate Tautomerase Trimer. Biochemistry, 58(22), 2617-2627.

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Protein Purification Techniques

Cited 4 times

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All chemicals, biochemical, and enzyme substrates were obtained from sources described elsewhere.^{7 ,8 (link),1 (link),13 (link),14 (link)} Chromatographic resins (DEAE Sepharose fast flow and Phenyl-Sepharose 6 fast flow) and columns (PD-10 Sephadex G-25M, Econo-Column, and Spectra/Chrom LC) were purchased from commercial vendors, as described.⁷ The Amicon stirred cell concentrators and the ultrafiltration membranes (10,000 Da, MW cutoff) were purchased from EMD Millipore Inc. (Billerica, MA). Coomassie Blue R-250 dye for staining sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) gels was adapted from a literature source.¹⁵

Medellin B.P., Lancaster E.B., Brown S.D., Rakhade S., Babbitt P.C., Whitman C.P, & Zhang Y.J. (2020). Structural Basis for the Asymmetry of a 4-Oxalocrotonate Tautomerase Trimer. Biochemistry, 59(16), 1592-1603.

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