Aerial parts of L. tuberosus were collected in Transylvania, Romania, in July 2020. Samples were dried at room temperature and stored in the dark. The voucher specimen of the plant labelled with a unique code was deposited at the Department of Pharmacognosy, University of Pécs, Pécs, Hungary (Voucher code: HV_10). The plant name follows the terminology of The World Flora Online (WFO, 2023).
For UHLC measurements, the aqueous and 50% (v/v) ethanolic extracts of the leaf were obtained by extracting 3.0 g of leaf powder in 30 mL of distilled water or 50% (v/v) ethanol using an ultrasonic bath (Bandelin Sonorex Digitec DT 1028, Berlin, Germany) three times, 30 min each (nominal ultrasonic power: 300 W, ultrasound frequency: 35 kHz). The extracts were distilled to dryness under reduced pressure with a rotary evaporator (Büchi Rotavapor R-200, Flawil, Switzerland) at 45 °C. The residues were dissolved in 20 mL of 70% (v/v) HPLC grade methanol and filtered through Minisart RC 15 0.2 µm syringe filters (Sartorius AG, Goettingen, Germany).
For antioxidant, antimicrobial and cellular measurements, plant extraction was conducted following our previous methodology without modifications [3 (link)].
For UHLC measurements, the aqueous and 50% (v/v) ethanolic extracts of the leaf were obtained by extracting 3.0 g of leaf powder in 30 mL of distilled water or 50% (v/v) ethanol using an ultrasonic bath (Bandelin Sonorex Digitec DT 1028, Berlin, Germany) three times, 30 min each (nominal ultrasonic power: 300 W, ultrasound frequency: 35 kHz). The extracts were distilled to dryness under reduced pressure with a rotary evaporator (Büchi Rotavapor R-200, Flawil, Switzerland) at 45 °C. The residues were dissolved in 20 mL of 70% (v/v) HPLC grade methanol and filtered through Minisart RC 15 0.2 µm syringe filters (Sartorius AG, Goettingen, Germany).
For antioxidant, antimicrobial and cellular measurements, plant extraction was conducted following our previous methodology without modifications [3 (link)].