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Pierce starting block pbs blocking buffer

Manufactured by Thermo Fisher Scientific
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Pierce Starting Block (PBS) Blocking Buffer is a ready-to-use solution designed to block non-specific binding sites in immunoassays and Western blotting applications. It is a phosphate-buffered saline (PBS) formulation that helps reduce background signal and improve the specificity of target protein detection.

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Lab products found in correlation

Ne per nuclear and cytoplasmic extraction reagent, by Thermo Fisher Scientific (4 mentions) Nupage lds sample buffer, by Thermo Fisher Scientific (2 mentions) Nupage bis tris gel, by Thermo Fisher Scientific (2 mentions) Trans blot turbo rta mini lf pvdf transfer kit, by Bio-Rad (2 mentions) Ibright western blot imaging system, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

PBS blocking buffer Block-buffer PBS buffer Blocking buffer

2 protocols using pierce starting block pbs blocking buffer

1

Protein Fractionation and Western Blot

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extractions were performed as previously described (Wohlhieter et al., 2020 (link)). Extraction of separate cytoplasmic and nuclear protein fractions were performed using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) according to the manufacturer’s protocol. Briefly, 30–50 μg of protein were mixed with NuPAGE LDS sample buffer (Invitrogen), loaded in a Bis-Tris Gel (NuPAGE, Invitrogen) and resolved. Electrophoresed protein samples were transferred with Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad, Alfred Nobel Drive Hercules, CA) for chemiluminescent detection. After incubating with Pierce Starting Block (PBS) Blocking Buffer (Thermo Fisher) at room temperature for 30 min, membranes were incubated in the primary antibodies (1:1000) overnight (the antibodies’ information are in Key resources table). Secondary anti-rabbit, horseradish peroxidase-linked antibodies were purchased from CST (#7074) and detected using iBright Western Blot Imaging Systems (Thermo Fisher).
Taniguchi H., Caeser R., Chavan S.S., Zhan Y.A., Chow A., Manoj P., Uddin F., Kitai H., Qu R., Hayatt O., Shah N.S., Villalonga Á.Q., Allaj V., Nguyen E.M., Chan J., Michel A.O., Mukae H., de Stanchina E., Rudin C.M, & Sen T. (2022). WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell reports, 39(7), 110814.
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2

Protein Fractionation and Western Blot

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extractions were performed as previously described (Wohlhieter et al., 2020 (link)). Extraction of separate cytoplasmic and nuclear protein fractions were performed using NE-PER™ Nuclear and Cytoplasmic Extraction Reagents (Thermo Fisher) according to the manufacturer’s protocol. Briefly, 30–50 μg of protein were mixed with NuPAGE LDS sample buffer (Invitrogen), loaded in a Bis-Tris Gel (NuPAGE, Invitrogen) and resolved. Electrophoresed protein samples were transferred with Trans-Blot Turbo RTA Mini LF PVDF Transfer Kit (Bio-Rad, Alfred Nobel Drive Hercules, CA) for chemiluminescent detection. After incubating with Pierce Starting Block (PBS) Blocking Buffer (Thermo Fisher) at room temperature for 30 min, membranes were incubated in the primary antibodies (1:1000) overnight (the antibodies’ information are in Key resources table). Secondary anti-rabbit, horseradish peroxidase-linked antibodies were purchased from CST (#7074) and detected using iBright Western Blot Imaging Systems (Thermo Fisher).
Taniguchi H., Caeser R., Chavan S.S., Zhan Y.A., Chow A., Manoj P., Uddin F., Kitai H., Qu R., Hayatt O., Shah N.S., Villalonga Á.Q., Allaj V., Nguyen E.M., Chan J., Michel A.O., Mukae H., de Stanchina E., Rudin C.M, & Sen T. (2022). WEE1 inhibition enhances the antitumor immune response to PD-L1 blockade by the concomitant activation of STING and STAT1 pathways in SCLC. Cell reports, 39(7), 110814.
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