Fibrin staining in the lung was carried out in inflation-fixed and paraffin-embedded lung sections. Immunostaining for fibrin was performed using a polyclonal rabbit anti-human fibrinogen (DAKO; Carpinteria, CA) at a 1:2000 dilution for 60 minutes. Rabbit IgG control (DAKO) was used at the same specifications and served as a negative control. The stains were developed using the peroxidase-based Envision detection system (DAKO). The counterstaining was performed using hematoxylin.
Rabbit anti human fibrinogen
Manufactured by Agilent Technologies
Sourced in United States
Rabbit anti-human fibrinogen is a polyclonal antibody produced by immunizing rabbits with purified human fibrinogen. It is designed for use in various immunoassay and research applications that require the detection or quantification of human fibrinogen.
Automatically generated - may contain errors
Lab products found in correlation
Rabbit igg control, by Agilent Technologies (1 mentions)
Envision detection system, by Agilent Technologies (1 mentions)
Hrp conjugated anti mouse igg, by GE Healthcare (1 mentions)
Rat anti mouse ly6g, by BD (1 mentions)
Annexin 5 cy5, by BD (1 mentions)
Phospho mapk family antibody sampler kit, by Cell Signaling Technology (1 mentions)
Horseradish peroxidase conjugated goat anti rabbit igg antibody, by Medical & Biological Laboratories (1 mentions)
3 protocols using rabbit anti human fibrinogen
1
Fibrin Immunostaining in Paraffin Lung Sections
2
Platelet Activation and Apoptosis Analysis
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Apyrase (Grade III, from potatoe). Cy™5 Annexin V (#559933) and mouse anti-human CD42a (#558819), FITC hamster anti mouse CD45 (#553252) and rat anti mouse Ly-6G (#551459) were from BD Biosciences. FITC hamster anti mouse CD40L (#ab24934) was from abcam, and FITC Mouse anti human FasL (#ab87023) was from abcam, PE rat anti mouse JON/A (integrin αIIbβ3, M023-2), FITC rat anti mouse CD62P (#M130-1), PE rat anti mouse GPIbα (##M040-2) and purified rat anti mouse GPIbα (#M042-0) was purchased from emfret analytics, cleaved caspase 3 (#9661), (phospho) MAPK family antibody sampler kit (#9926 and #9910) and (phospho) rabbit anti mouse MEK1/2 (#9122, #9121) was from Cell Signaling, rabbit anti mouse EGR-1 (#sc-110) was from Santa Cruz, rabbit anti human fibrin(ogen) (#2022-03) was from Dako, GP9 antibody was purchased from Bioorbyt (#orb 167288) and HRP-conjugated anti-mouse IgG (#NA931) was from GE Healthcare. All other reagents were of analytical grade.
3
Protein Characterization by SDS-PAGE and Western Blot
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The characterization of proteins was performed by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) in reducing conditions (10% polyacrylamide gel) and non-reducing conditions (7% polyacrylamide gel). These gels were treated by Coomassie Brilliant Blue R-250 staining or western blotting analysis developed using rabbit anti-human fibrinogen (DAKO, Carpinteria, CA, USA), antihuman IgA (α-chain), anti-human kappa light chain (Medical and Biological Laboratories Ltd), and anti-human lambda light chain (Medical and Biological Laboratories Ltd) antibodies, and horseradish peroxidase-conjugated goat anti-rabbit IgG antibody (Medical and Biological Laboratories Ltd). The blots were visualized using 3,3'-diaminobenzidine (DAB) and hydrogen peroxide.
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