Tpl2 kinase inhibitor

Manufactured by Santa Cruz Biotechnology

Tpl2 kinase inhibitor is a chemical compound designed to selectively inhibit the activity of the Tpl2 (Tumor Progression Locus 2) kinase enzyme. Tpl2 kinase is involved in various cellular signaling pathways and plays a role in the regulation of inflammation and immune responses. The Tpl2 kinase inhibitor is a tool used in research to study the specific functions and effects of the Tpl2 kinase in biological systems.

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Lab products found in correlation

Anti cd49a ha31 8, by BD (1 mentions) Bms 345541, by Merck Group (1 mentions) Pyr 41, by Merck Group (1 mentions) Anti ifnγ xmg1.2, by Thermo Fisher Scientific (1 mentions) Cgp57380, by Merck Group (1 mentions) Isrib, by Merck Group (1 mentions) Mg132, by Cell Signaling Technology (1 mentions) Actinomycin d, by Merck Group (1 mentions) Cycloheximide (chx), by Merck Group (1 mentions) Easysep mouse nk cell isolation kit, by STEMCELL (1 mentions) U0126, by Santa Cruz Biotechnology (1 mentions)

2 protocols using tpl2 kinase inhibitor

NK Cell Isolation and Characterization

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Fluorescent-labeled antibodies used were anti-NK1.1 (clone PK136), anti-NKp46 (29A1.4), anti-CD3 (145–2C11), anti-CD19 (eBio1D3), anti-CD45.1 (A20), anti-CD45.2(104), anti-Eomes (Dan11mag), anti-TNFα (MP6-XT22), and anti-IFNγ (XMG1.2), all from Thermo Fisher Scientific. Fluorescent-labeled anti-CD49a (Ha31/8) was purchased form BD Biosciences. Biotinylated anti-Ly49H (3D10) was produced in-house. MG-132(Cell Signaling 2094S), Actinomycin D (Sigma A9415), Cycloheximide (Sigma C7698), CGP-57380 (Sigma C0993), ISRIB (Sigma SML0843), Pyr-41 (Sigma N2915), BMS-345541 (Sigma B9935), Tpl2 kinase inhibitor (Santa Cruz sc-204351), PD90859 (Sigma P215) were used at the indicated concentrations. Where indicated, NK cells were enriched to a purity >90% NK1.1+CD3-CD19- cells, verified by flow cytometry for each experiment, from RAG1^−/− splenocytes using CD49b microbeads with LS columns (Miltenyi Biotec) or from C57BL/6 mice using EasySep Mouse NK cell isolation kit (StemCell Technologies) according to manufacturer’s instructions. m157-Tg or WT murine embryonic fibroblasts (MEFs) were isolated from day 11.5–13.5 embryos.

Piersma S.J., Pak-Wittel M.A., Lin A., Plougastel-Douglas B, & Yokoyama W.M. (2019). Activation Receptor-dependent IFNγ Production by NK Cells is Controlled by Transcription, Translation and the Proteasome. Journal of immunology (Baltimore, Md. : 1950), 203(7), 1981-1988.

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Kinase Inhibition Experimental Protocol

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Tpl2 Kinase Inhibitor (Santa Cruz, sc-204351), U-0126 (Santa Cruz, sc-222395), and SB 203580 (Calbiochem, 559389) were used in this study. All kinase inhibitors were dissolved in 100% DMSO, and DMSO was used as the vehicle control treatment for all experiments.

Chowdhury F.Z., Estrada L.D., Murray S., Forman J, & Farrar J.D. (2014). Pharmacological Inhibition of TPL2/MAP3K8 Blocks Human Cytotoxic T Lymphocyte Effector Functions. PLoS ONE, 9(3), e92187.

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