Pluripotent stem cell sfm xf ff medium

Manufactured by American Type Culture Collection

Pluripotent Stem Cell SFM XF/FF medium is a serum-free and animal component-free culture medium designed for the maintenance and expansion of human pluripotent stem cells, including embryonic stem cells and induced pluripotent stem cells, in an undifferentiated state.

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Lab products found in correlation

Essential 8 flex medium, by Thermo Fisher Scientific (2 mentions) Relesr, by STEMCELL (1 mentions) Vtn n, by Thermo Fisher Scientific (1 mentions) Cellmatrix basement membrane gel, by American Type Culture Collection (1 mentions) Mtesr1 medium, by STEMCELL (1 mentions)

2 protocols using pluripotent stem cell sfm xf ff medium

Maintenance of Three Distinct hiPSC Lines

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Three distinct hiPSC lines were used in the current study: STAN, ATCC, and BJFF. STAN line was purchased from WiCell (#STAN061i-164-1), ATCC line was acquired from ATCC (#ATCCACS-1019), and BJFF was obtained from the Genome Engineering and iPSC Core at Washington University in Saint Louis. All three lines were reprogrammed by Sendai virus from human foreskin fibroblasts and confirmed to be karyotypically normal and mycoplasma free. STAN and BJFF hiPSCs were maintained on vitronectin coated 6-well plates (Thermo Fisher Scientific, #A31804) in Essential 8 Flex medium (Thermo Fisher Scientific, #A2858501). ATCC hiPSCs were cultured on CellMatrix Basement Membrane Gel coated 6-well plates (ATCC, #ACS3035) in Pluripotent Stem Cell SFM XF/FF medium (ATCC, #ACS3002). Cells were fed daily and passaged with ReLeSR (STEMCELL Technologies, #05872). All hiPSC lines were maintained below passage 30.

Wu C.L., Dicks A., Steward N., Tang R., Katz D.B., Choi Y.R, & Guilak F. (2021). Single cell transcriptomic analysis of human pluripotent stem cell chondrogenesis. Nature Communications, 12, 362.

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Characterization of Three hiPSC Lines

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Three human iPSC lines were used in the current study: BJFF.6,
ATCC®ACS-1019 and RVR-iPSC.[54 (link),60 (link)] Both BJFF.6 (BJFF) and
ATCC®ACS-1019 (ATCC) were derived from foreskin fibroblasts of male
newborn infants using sendai virus. BJFF was obtained from iPSC core at
Washington University in St. Louis, and the cells were maintained on vitronectin
(VTN-N; Fisher Scientific, Hampton NH) coated plates in Essential 8 Flex medium
(E8; Life Technologies, Carlsbad CA). The ATCC line was purchased from ATCC
(Manassas VA), and the cells were maintained on CellMatrix Basement Membrane Gel
(ATCC) in Pluripotent Stem Cell SFM XF/FF medium (ATCC). RVR-iPSCs were
retrovirally reprogrammed from BJ fibroblasts and characterized
previously.[60 (link)] The cells were
maintained on vitronectin in mTeSR1 medium (Stemcell Technologies, Vancouver
CA). Media were changed daily and cells were passaged at 80–90%
confluency.

Adkar S.S., Wu C.L., Willard V.P., Dicks A., Ettyreddy A., Steward N., Bhutani N., Gersbach C.A, & Guilak F. (2018). Step-Wise Chondrogenesis of Human Induced Pluripotent Stem Cells and Purification Via a Reporter Allele Generated by CRISPR-Cas9 Genome Editing. Stem cells (Dayton, Ohio), 37(1), 65-76.

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