Ifn γ alexa fluor 700 b27

Manufactured by BD

The IFN-γ Alexa Fluor 700 (B27) is a fluorescently-labeled antibody used for the detection and quantification of interferon-gamma (IFN-γ) in various biological samples. The antibody is conjugated with Alexa Fluor 700, a fluorescent dye with excitation and emission wavelengths of 702 nm and 723 nm, respectively. This product can be used in flow cytometry and other immunoassay applications for the analysis of IFN-γ expression.

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Lab products found in correlation

Cd3 bv650 okt3, by BD (1 mentions) Cd4 percp cy5.5 l200, by BD (1 mentions) Iotest beta mark tcr vβ repertoire kit, by Beckman Coulter (1 mentions) Live dead fixable dead cell stain kit, by Thermo Fisher Scientific (1 mentions) Cd4 alexa700, by BD (1 mentions) Ki67 fitc, by BD (1 mentions) Cd8 fitc bw135 80, by Miltenyi Biotec (1 mentions) Cd19 fitc lt19, by Miltenyi Biotec (1 mentions) Ccr4 pe cy7 1g1, by BD (1 mentions) Cd45ra apc cy7, by BD (1 mentions)

Close spelling variants of this product

IFN-γ (389 citations) Alexa Fluor 700 (17 citations) IFN-γ (B27)( (9 citations)

2 protocols using ifn γ alexa fluor 700 b27

Characterizing M. tuberculosis-specific T cells

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The IOTest Beta Mark TCR Vβ repertoire kit (Beckman Coulter) was used to determine the TCR Vβ repertoire of M. tuberculosis–specific IFN-γ– or IL-22–producing CD4⁺ T cells. Briefly, cells from M. tuberculosis lysate-stimulated whole blood were stained with CD3 BV650 (OKT3; BD Biosciences), CD4 PerCP-Cy5.5 (L200; BD Biosciences), IFN-γ Alexa Fluor 700 (B27; BD Biosciences), IL-22 allophycocyanin (22JOP; eBioscience), and each of the eight vials containing mixtures of conjugated TCR Vβ Abs corresponding to 24 different specificities.

Makatsa M.S., Omondi F.M., Bunjun R., Wilkinson R.J., Riou C, & Burgers W.A. (2022). Characterization of Mycobacterium tuberculosis–Specific Th22 Cells and the Effect of Tuberculosis Disease and HIV Coinfection. The Journal of Immunology Author Choice, 209(3), 446-455.

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Multiparametric Flow Cytometry Analysis

Cited 1 time

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Fluorochrome-conjugated Abs used for polychromatic flow cytometry analysis were CD3-Pacific Blue (UCHT1), CD4-Alexa700 (RPA-T4), CD45RA-APC-Cy7 (custom), CCR4-PE-Cy7 (1G1), CXCR3-PE-Cy5 (1C6), CCR6-PE (11A9), Ki67-FITC, IFN-γ-AlexaFluor 700 (B27) (BD Pharmingen), CD56-FITC (MEM188), IL-17-PE (64DEC17) (eBioscience), HIV-p24-FITC (FH190-1-1) (Beckman Coulter), CD8-FITC (BW135/80), and CD19-FITC (LT19) (Miltenyi). A viability dye (Molecular Probes^® LIVE/DEAD^® Fixable Dead Cell Stain Kits, Invitrogen) was used to exclude dead cells. Cells were stained and analyzed by FACS using the BD LSRII cytometer and the FlowJo software, as previously described [129 (link)].

Cleret-Buhot A., Zhang Y., Planas D., Goulet J.P., Monteiro P., Gosselin A., Wacleche V.S., Tremblay C.L., Jenabian M.A., Routy J.P., El-Far M., Chomont N., Haddad E.K., Sekaly R.P, & Ancuta P. (2015). Identification of novel HIV-1 dependency factors in primary CCR4+CCR6+Th17 cells via a genome-wide transcriptional approach. Retrovirology, 12, 102.

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