Crispr cas9 technique
The CRISPR/Cas9 technique is a gene-editing tool that allows for precise modification of DNA sequences. It utilizes a guide RNA and the Cas9 enzyme to target and edit specific genetic regions.
Lab products found in correlation
5 protocols using crispr cas9 technique
Generation and Characterization of Trim40 Knockout Mice
BALB/c and TLR4 KO Mice DNA Vaccination
Establishing and Characterizing LBH-Knockout Mouse Model for Myocardial Infarction
Generation and Validation of Tmem150b Knockout Mice
Generation of Knock-in Mouse Models
Ryr2N1552S/+, Ryr2R137W/+, Plxnb2D1577E/+, and Plxnb2R465Q/+ knock-in mice were constructed by the CRISPR/Cas9 technique (Cyagen, Suzhou, China). The gRNAs, donor oligos containing each mutant, to Plxnb2 (p.D1577E and p.R465Q) or Ryr2 (p.N1552S and p.R137W) gene, and Cas9 was coinjected into fertilized mouse eggs to generate targeted knock-in offspring. Genotyping was performed by PCR from genomic DNA of mouse tails followed by Sanger sequencing. The method is shown in
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!