Antibodies for erα

Apigenin and histamine were from Sigma-Aldrich (St. Louis, MO, USA). The antibodies against Bax (1:1000), PI3K (1:1000), Akt (1:1000), and mTOR (1:1000) were obtained from Abbkine Scientific. (Wuhan, China). Antibodies for ERα (1:500), ERβ (1:500), p-PI3K (1:1000) and p-Akt (1:5000) were purchased from Abcam (Burlingame, city, CA, USA). AZD9496 (CAS No.: 1639042-08-2), PHTPP (CAS No.: 805239-56-9), PPT (CAS No.: 263717-53-9) and DPN (CAS No.: 1428-67-7) were obtained from MedChemExpress (Shanghai, China). FBS (Fetal bovine serum) was from Hyclone (Logan, UT, USA). Dulbecco’s modified Eagle medium (DMEM) was purchased from Gibco (Grand Island, NY, USA).

API, E2 were obtained from Sigma-Aldrich (St. Louis, MO, USA). Mouse anti-DNP-IgE monoclonal antibody, DNP-HSA, ovalbumin (OVA), and cholera toxin (CT) were obtained from Sigma-Aldrich. Antibodies for ERα (1:500) and ERβ (1:500) were purchased from Abcam (Burlingame, CA, USA). Mouse IL-4, TNF-α, MCT-1, and histamine ELISA kit were from Dongge Weiye (Beijing, China). Rabbit-derived phosphorylated ERK1/2 monoclonal antibody, rabbit-derived phosphorylated JNK polyclonal antibody, rabbit-derived phosphorylated PLCγ polyclonal antibody was obtained from Lianke Biotechnology (Hangzhou, China). Dulbecco’s modified Eagle medium (DMEM) was purchased from Gibco (Grand Island, NY, USA).