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Model 8451a

Manufactured by Hewlett-Packard
Sourced in United States

The Model 8451A is a UV-Visible Spectrophotometer manufactured by Hewlett-Packard. It is a laboratory instrument designed for the measurement of absorption and transmission spectra in the ultraviolet and visible wavelength ranges.

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3 protocols using model 8451a

1

Synthesis of Heterocyclic Compounds

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Starting materials were purchased from Aldrich
Chemical Co. (Milwaukee, WI) unless otherwise indicated. NMR spectra
were recorded on a Varian UNITY INOVA spectrometer. Chemical shifts
(δ) are reported in parts per million (ppm) downfield from tetramethylsilane.
Ultraviolet (UV) spectra were recorded on an Agilent model 8453 or
a Hewlett-Packard model 8451A diode array spectrophotometer. Elemental
analyses were performed by Midwest Microlab (Indianapolis, IN). Chromatography
was performed on a Biotage SP1 flash purification system. Prepacked
silica gel flash chromatography columns were purchased from Silicycle
(Quebec City, Canada) or from Yanazen Science Inc. (San Bruno, CA).
Compounds 1,10 (link)2,22 (link)6a,10 (link)6b,10 (link) and 8(11 (link)) were prepared as previously
described. In addition to NMR, UV, and HPLC with HRMS, CHN combustion
analysis was performed to determine purity of compounds. All compounds
were ≥95% pure.
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2

Transparent Film Characterization by UV-Vis

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The transmittance and transparency of the films were estimated using UV-vis spectroscopy, according to a prior study [42 (link),43 (link),57 (link)]. In brief, the films were cut into pieces of 1 × 2 cm2, and their transmittance was recorded at 600 nm using a UV-vis spectrophotometer (Model 8451A, Hewlett-Packard Co., USA). The results were presented as transmittance percentage (T600 %), and the film transparency (T) was calculated from Equation (1) [57 (link)]:
where T600 is a fraction expressing the amount of light transmitted through the film and t is the film thickness in mm.
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3

Biofilm Transparency Measurement by UV-Vis

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Another essential property of biofilms is their transparency, which restricts light transmission while allowing visibility through the packaging material. To determine this property, UV–vis spectroscopy was used. Thus, 1 × 2 cm2 samples were measured in a UV–vis spectrophotometer (Model 8451A, Hewlett Packard Co., Santa Clara, CA, USA) at 600 nm. A blank was carried out with air [24 (link),38 (link)]. The results were indicated as transmittance (amount of light that can pass through the system).
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