Purified starch (30 mg for fractionation of rice starch and 20 mg for corn starch) was suspended in 1.6 mL of distilled water in a 15 mL centrifuge tube, and gelatinized with 400 µL of 5 M NaOH for 30 min at 37 °C. Distilled water (2 mL) and 2 mL of eluent (0.2 % NaCl / 0.05 M NaOH) were added, and samples were filtered through a 5 µm Durapore polyvinylidene fluoride (PVDF) membrane (Merck KGaA, Darmstadt, Germany). A 5 mL sample of filtrate was applied to a 4-column system comprising Toyopearl HW75S × 2, HW65S, and HW55S columns (2.2 cm diameter, 30 cm × 4; Tosoh Corp., Tokyo, Japan) pre-equilibrated with 0.2 % NaCl / 0.05 M NaOH, and carbohydrate was detected using a RI-8020 RI detector (Tosoh Corp.).16) Columns were incubated at 40 °C, and samples were eluted at a flow rate of 1 mL/min by using a DP-8020 pump (Tosoh Corp.).
Durapore polyvinylidene fluoride pvdf membrane
Manufactured by Merck Group
Sourced in Germany
Durapore polyvinylidene fluoride (PVDF) membrane is a type of lab equipment used for filtration and separation purposes. It is a hydrophilic polymer-based membrane that is chemically and thermally stable. The membrane is designed to provide efficient and reliable performance in various laboratory applications.
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2 protocols using durapore polyvinylidene fluoride pvdf membrane
1
Fractionation of Starch Polymers
2
Rice Flour Carbohydrate Analysis
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Rice flour samples (20 mg) were suspended in 1.6 mL of distilled water in a 15 mL centrifuge tube and gelatinized with 400 µL of 5 M NaOH for 30 min at 37 °C. Then, 2 mL of distilled water and 2 mL of eluent (0.2% NaCl/0.05 M NaOH) were added to each sample, followed by filtration through a 5 µm Durapore polyvinylidene fluoride (PVDF) membrane (Merck, Darmstadt, Germany). A 5 mL aliquot of the filtrate was used in a 4-column system (Toyopearl HW75S × 2-HW65S-HW55S; 2.2 cm diameter, 30 cm × 4; Tosoh Corp., Tokyo, Japan) that was pre-equilibrated with the eluent, and carbohydrates were detected using an RI detector (RI-8020; Tosoh Corp.). The columns were incubated at 40 °C, and the samples were eluted at a flow rate of 1 mL/min using a DP-8020 pump (Tosoh Corp.).
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