Double sided pressure sensitive adhesive tape 200 mp 7956mp

Polyvinyl chloride (PVC) films of 180 μm thickness were bought at Delbo (Maldegem, Belgium). Double-sided pressure-sensitive adhesive (PSA) tape (200 MP 7956MP) and transfer tape (467MP) with respective thicknesses of 153 and 50 μm were acquired from 3M (Minnesota, USA). Microfluidic dyes were obtained from Darwin Microfluidics (Paris, France). Hydrophobic solutions Aquapel and Fluoropel 800 M were purchased at Aquapel (Pennsylvania, USA) and Cytonix (Maryland, USA), respectively. Whatman quantitative filter paper of grades 43 and 598, fluorescein, bovine serum albumin (BSA) and phosphate buffer saline (PBS) were all obtained from Sigma-Aldrich (Overijse, Belgium). Tween 20 was purchased at Promega (Madison, USA). Left-over EDTA whole blood samples were obtained from the Red Cross Flanders and used within 48 h after receipt.

Whatman quantitative filter paper grades 43 and 598 (with respective thicknesses of 220 and 320 µm), Whatman 903 protein saver cards, sulphuric acid (H 2 SO 4 ) and horseradish peroxidase (HRP) were obtained from Sigma-Aldrich (Belgium). Whatman qualitative filter paper grade CF12 was purchased from GE Healthcare (Belgium). Merck (Germany) delivered the peroxide (H 2 O 2 ) and the o-phenylenediamine was obtained from Acros Organics (Belgium). PVC plastic films of 180 µm thickness were bought from Delbo (Belgium). Double-sided pressure-sensitive adhesive (PSA) tape (200MP 7956MP) and transfer tape (467MP) were acquired from 3M (USA). Aquapel and Fluoropel 800M hydrophobic agents were purchased from Aquapel (USA) and Cytonix (USA), respectively. Adalimumab (clone DE27) was acquired from Bio-rad laboratories (Belgium). Superblock™ T20 (PBS) blocking buffer was bought at Thermofisher Scientific (Germany). Monoclonal anti-ADM capture antibodies (MA-ADM28B8) and HRP-conjugated anti-ADM detection antibodies (MA-ADM40D8) were generated inhouse at the Laboratory for Therapeutic and Diagnostic Antibodies (KU Leuven, Belgium) 33 .