The prepared tissue sections were briefly rinsed in 0.1 M phosphate-buffered saline (PBS) and incubated with 0.1% Triton X-100 for 30 min. Subsequently, the sections were blocked with 0.1 M PBS containing 0.03% Triton X-100 and 5% goat serum for 1 h. The primary antibodies were anti-IFITM3 (Abcam, ab15592, 1:100), anti-glial fibrillary acidic protein (GFAP) (Abcam, ab4648, 1:200), anti-neuronal nuclear protein (NeuN) (Abcam, ab104224, 1:500), and ionized calcium-binding adapter molecule 1 (Iba1) (Novus Biologicals, NB-1028ss, 1:100). The tissue sections were incubated for 2 h at room temperature of 24°C, kept overnight at 4°C, and washed three times with PBS for 5 min. They were incubated in the dark with Alexa Fluor 488-conjugated goat anti-rabbit (Abcam, 1:300) or Alexa Fluor 594-conjugated goat anti-mouse (Abcam, 1:300) secondary antibody dilutions for 2 h at room temperature, respectively. They were washed three times with PBS for 5 min. The slides were mounted in glycerol, observed under a fluorescence microscope, and photographed.
Nb 1028ss
Manufactured by Novus Biologicals
The NB-1028ss is a stainless steel autoclavable tube roller mixer. It is designed to provide gentle, uniform mixing of samples in test tubes or other containers. The tube roller mixer operates at a constant speed to ensure consistent sample agitation.
Automatically generated - may contain errors
2 protocols using nb 1028ss
1
Immunofluorescence Labeling of Brain Markers
2
Immunofluorescence Labeling of Brain Markers
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The prepared tissue sections were briefly rinsed in 0.1 M phosphate-buffered saline (PBS) and incubated with 0.1% Triton X-100 for 30 min. Subsequently, the sections were blocked with 0.1 M PBS containing 0.03% Triton X-100 and 5% goat serum for 1 h. The primary antibodies were anti-IFITM3 (Abcam, ab15592, 1:100), anti-glial fibrillary acidic protein (GFAP) (Abcam, ab4648, 1:200), anti-neuronal nuclear protein (NeuN) (Abcam, ab104224, 1:500), and ionized calcium-binding adapter molecule 1 (Iba1) (Novus Biologicals, NB-1028ss, 1:100). The tissue sections were incubated for 2 h at room temperature of 24°C, kept overnight at 4°C, and washed three times with PBS for 5 min. They were incubated in the dark with Alexa Fluor 488-conjugated goat anti-rabbit (Abcam, 1:300) or Alexa Fluor 594-conjugated goat anti-mouse (Abcam, 1:300) secondary antibody dilutions for 2 h at room temperature, respectively. They were washed three times with PBS for 5 min. The slides were mounted in glycerol, observed under a fluorescence microscope, and photographed.
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