Trizol reagent

Manufactured by Selleck Chemicals

TRIzol reagent is a monophasic solution of phenol, guanidine isothiocyanate, and other proprietary components designed for the isolation of total RNA from various biological samples. It is a versatile reagent that can be used for the isolation of RNA, DNA, and proteins from a single sample.

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Lab products found in correlation

2 protocols using trizol reagent

Ferroptosis Induction in A549 Cells

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A549 cells were treated in triplicate with 0.7% dimethyl sulfoxide (DMSO; solvent used to dissolve RSL3 and Fer-1), 2 μM RSL3 (28 (link)) (cat. no. HY-100218A; MedChemExpress), or 2 μM RSL3 and 5 μM Fer-1 (29 (link)) (cat. no. S7243; Selleck Chemicals) for 24 h. Total RNA was extracted using TRIzol reagent (cat. no. T9424; Sigma-Aldrich; Merck KGaA), and the quantity and purity of the extracted RNA were analysed using an RNA 6000 Nano LabChip Kit (cat. no. 5067-1511; Agilent Technologies, Inc.) by Bioanalyzer 2100 (Agilent Technologies, Inc.). The 2x 150 bp paired-end sequencing was performed using an Illumina Novaseq™ 6000 at LC-Bio Technology (Hangzhou) Co., Ltd.

Tai F., Zhai R., Ding K., Zhang Y., Yang H., Li H., Wang Q., Cao Z., Ge C., Fu H., Xiao F, & Zheng X. (2024). Long non-coding RNA lung cancer-associated transcript 1 regulates ferroptosis via microRNA-34a-5p-mediated GTP cyclohydrolase 1 downregulation in lung cancer cells. International Journal of Oncology, 64(6), 64.

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Platelet Activation and Signaling Pathway

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TPO (Cat. no. 1624) was purchased from Tocris (Minneapolis, MN, USA). JYBL-1 was from Sigma-Aldrich (Solarbio Life Sciences), and TRIzol^® reagent was purchased from Selleckchem (Invitrogen). SYBR™ Premix Ex Taq II was purchased from Thermo Fisher Scientific (Waltham USA, MA). CREG1 was purchased from Sigma-Aldrich (09015-1B7) and HUABIO (ER61836). The DyLight™ 488 conjugation kit, and anti-CD42a, anti-CD42b, and anti-CD41 antibodies, were purchased from Abcam (Cambridge, USA). Anti-GAPDH, anti-cleaved caspase-3, anti-phospho-MEK1/2, anti-MEK1/2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-ERK1/2, anti-p38, anti-phospho-p38 (Thr180/Tyr182), anti-phospho-PAK1/2 (Ser209), anti-phospho-LIMK1, anti-phospho-cofilin, anti-total PAK, anti-cofilin, and anti-total LIMK1 antibodies were obtained from Cell Signaling Technology (Danvers, MA, USA). PE-CD41 was obtained from BD Biosciences, and FITC-CD42b was obtained from Emfret Analytics. U0126 and propidium iodide were obtained from Sangon Biotech (Shanghai, China).

Song H., Li J., Peng C., Liu D., Mei Z., Yang Z., Tian X., Zhang X., Jing Q., Yan C, & Han Y. (2023). The role of CREG1 in megakaryocyte maturation and thrombocytopoiesis. International Journal of Biological Sciences, 19(11), 3614-3627.

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