Antibodies against NQO1 (11451-1-AP), Nox1 (17772-1-AP), Bax (50599-2-IG), Nox4 (14347-1-AP), Sirt1 (13161-1-AP) and β‐actin (20536-1-AP) were purchased from Proteintech (Chicago, IL, USA). 8-Hydroxy-2'-deoxyguanosine (8-OHdG) (ab48508) and Bcl-2 (ab32124) were purchased from Abcam (Cambridge, UK). The Cleaved Caspase-3 (9664) antibody was obtained from Cell Signaling Technology (Danvers, MA). The culture medium, fetal bovine serum (FBS) and Dulbecco's modified Eagle's medium (DMEM)-F12 were obtained from Gibco Company (Gaithersburg, MD). The plasmids for NQO1 pcDNA3.1(+) and control pcDNA3.1(+) were obtained from Gene Pharma (Shanghai, China). The transfection reagent FuGENE-HD was obtained from Promega (Madison, Wisconsin, USA). TRIzol was purchased from Invitrogen (Carlsbad, CA). Takara (Shiga, Japan) provided SYBR Premix Ex Taq II. EX527 was purchased from Selleckchem (Shanghai, China). A TUNEL FITC apoptosis detection kit was purchased from Vazyme Biotech Corporation (Nanjing, China). Adeno-associated virus serotype 9 (AAV9) was obtained from HanBio Technology (HH20191024HBYXL-AAV01, Shanghai, China).
Adeno associated virus serotype 9 aav9
Manufactured by Hanbio Biotechnology
Sourced in China
Adeno-associated virus serotype 9 (AAV9) is a type of laboratory equipment used in biotechnology research. AAV9 is a viral vector that can be used to deliver genetic material into target cells. Its core function is to serve as a tool for gene delivery and gene therapy applications.
Automatically generated - may contain errors
Lab products found in correlation
Sybr premix ex taq 2, by Takara Bio (2 mentions)
Trizol, by Thermo Fisher Scientific (2 mentions)
Dulbecco s modified eagle s medium dmem f12, by Thermo Fisher Scientific (1 mentions)
Fugene hd, by Promega (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
β actin 20536 1 ap, by Proteintech (1 mentions)
Ex527, by Selleck Chemicals (1 mentions)
Sirt1 13161 1 ap, by Proteintech (1 mentions)
Nf κb p65, by Abcam (1 mentions)
Fugene hd transfection reagent, by Promega (1 mentions)
Control pcdna3.1 plasmid, by GenePharma (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
E cadherin, by Proteintech (1 mentions)
Interleukin 6 (il 6), by Proteintech (1 mentions)
Tgf β1, by Abcam (1 mentions)
β actin, by Proteintech (1 mentions)
Dmem f12 medium, by Thermo Fisher Scientific (1 mentions)
α smooth muscle actin α sma, by Proteintech (1 mentions)
Tak 242, by Selleck Chemicals (1 mentions)
Tempol, by Selleck Chemicals (1 mentions)
2 protocols using adeno associated virus serotype 9 aav9
1
Antibody-based Assessment of Oxidative Stress
2
Antibodies and Reagents for Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
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2.1 Antibodies and other reagents DMEM, DMEM-F12 medium and fetal bovine serum (FBS) was from Gibco (Gaithersburg, Maryland). Penicillin and streptomycin were purchased from Invitgen (Carlsbad, CA). N-acetylcysteine (NAC), mannitol came from Sigma (St. Louis, MO). Tempol and TAK242 came from Selleck Chemicals (Houston, Texas). NQO1 pcDNA3.1(+) plasmid and control pcDNA3.1(+) plasmid were bought from GenePharma (Suzhou, China). FuGENEHD transfection reagents were obtained from Promega (Madison, Wisconsin, USA). TRIzol was bought from Invitrogen (Carlsbad, CA). Takara (Shiga, Japan) was the source of SYBR Premix Ex Taq II. The antibodies against α-smooth muscle actin (α-SMA), E-cadherin, collagen IV, bronectin, Nox1, Nox4, TLR4, NQO1, Histone H3, IL-6, TNF-α and β-actin were bought from Proteintech (Chicago, IL), while the antibodies of TGF-β1, NF-κB p65 and MCP-1 came from Abcam (Cambridge, UK). Antibodies against Smad-2, Smad-3, P-Smad2, P-Smad3 were purchased from Cell Signaling Technology (Beverly, MS). Adeno-associated virus serotype 9 (AAV9) was obtained from HanBio Technology (HH20191024HBYXL-AAV01, Shanghai, China).
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