Rat L6 myoblasts (CRL 1458) were purchased from ATCC (APABCAM, Rio de Janeiro Brazil). Dulbecco’s-modified Eagle’s medium (DMEM) and fetal bovine serum (FBS) were from Vitrocell Embriolife (Campinas, SP, Brazil). Antibiotic (penicillin/streptomycin), bovine serum albumin (BSA), palmitate (P5585) and 3-(4,5-dimethylthiazol-2-yI)-2,5-diphenyltetrazolium bromide (MTT) were obtained from Sigma (St. Louis, MO, EUA). Trizol, Platinum SYBR Green qPCR SuperMix UDG and Taqman primers and probes were obtained from Life Technologies (Carlsbad, CA, USA), ImProm-II Reverse Transcriptase from Promega (Madison, WI, USA). Anti-GLUT4 (#07–1404) antibody was obtained from Millipore (Billerica, MA, USA), and anti-phospho-IKK-A/B (#2681) from Cell Signaling (Beverly, MA, USA). Anti phospho-PERK (Thr 981) (sc-32577), anti-PERK (H-300) (sc-13073), anti- eIF-2A (FL-315) (sc-11386), anti- IRE1a (H-190) (sc-20790), anti-PR15A (C-19) (sc-825), anti-DDIT-3 (F-168) (sc-575), anti-TRAF2 (C-20) (sc-876), and anti-NFKB p50 subunit (C-19) (sc-1190) antibodies were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Anti-phospho-eIF-2A (Ser 51) (ab 32157), anti-XBP1 (ab 37152), anti-NFKB p65 subunit (ab 7970), and anti-GRP78 (ab 108615) antibodies were from Abcam (Cambridge, MA, USA), and horseradish peroxidase-linked immunoglobulin was from Amersham Biosciences (Buckinghamshire, UK).
Dulbecco s modified eagle s medium dmem
Manufactured by Vitrocell
Sourced in Brazil
Dulbecco's Modified Eagle's Medium (DMEM) is a cell culture medium commonly used to support the growth of various cell types, including mammalian cells. It provides essential nutrients, amino acids, vitamins, and other components required for cell maintenance and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Vitrocell (5 mentions)
Trypsin edta solution, by Merck Group (3 mentions)
Phosphate buffered saline (pbs), by Merck Group (3 mentions)
Amphotericin b, by Vitrocell (3 mentions)
L glutamine, by Vitrocell (3 mentions)
Gentamicin sulphate, by Vitrocell (2 mentions)
Chitosan, by Merck Group (2 mentions)
Gentamicin sulfate, by Vitrocell (2 mentions)
Taqman primers and probe, by Thermo Fisher Scientific (1 mentions)
Anti phospho ikka b, by Cell Signaling Technology (1 mentions)
Platinum sybr green qpcr supermix udg, by Thermo Fisher Scientific (1 mentions)
Trizol, by Thermo Fisher Scientific (1 mentions)
Improm 2 reverse transcriptase, by Promega (1 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (1 mentions)
Sodium chloride (nacl), by Merck Group (1 mentions)
Kh2po4, by Merck Group (1 mentions)
Na2hpo4, by Merck Group (1 mentions)
Rpmi 1640, by Vitrocell (1 mentions)
Pentasodium tripolyphosphate, by Merck Group (1 mentions)
Acetonitrile, by Tedia (1 mentions)
6 protocols using dulbecco s modified eagle s medium dmem
1
Rat L6 Myoblast Assay Protocol
2
Chitosan-Based Biomaterial Characterization
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Chitosan (CS) of low (LMW-CS) and medium molecular weight (MMW-CS) (deacetylation degree, 75-85%; and viscosity of 20-300 cP or 200-800 cP, respectively, according to the manufacturer's data sheet), pentasodium tripolyphosphate (TPP), 2,5-diphenyl-3,-(4,5dimethyl-2-thiazolyl) tetrazolium bromide (MTT), neutral red (NR) dye, dimethyl sulfoxide (DMSO), phosphate buffered saline (PBS) and trypsin-EDTA solution (170,000 U l-1 trypsin and 0.2 g l-1 EDTA) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Fetal bovine serum (FBS) and Dulbecco's Modified Eagle's Medium (DMEM), supplemented with Lglutamine (584 mg/l) and antibiotic/antimicotic (50 mg/ml gentamicin sulphate and 2 mg/l amphotericin B), were purchased from Vitrocell (Campinas, SP, Brazil). All other reagents were of analytical grade. NaCl, Na2HPO4 and KH2PO4 were from Merck (Darmstadt, Germany).
3
Culturing Human Breast Cancer Cells
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MCF-7 (moderately invasive) and MDA-MB 231 (highly metastatic), human mammary adenocarcinoma cell lines, and CHO, a non-tumor cell line derived from the ovary of the Chinese hamster, were obtained from the Rio de Janeiro Cell Bank (PABCAM, Federal University of Rio de Janeiro, RJ, Brazil). MCF-7, MDA-MB 231, and CHO cell cultures were maintained in RPMI1640, supplemented with 20% of fetal bovine serum (FBS), Leibovitz's supplemented with 10% FBS and Dulbecco's modified Eagle's medium (DMEM) supplemented with 10% FBS, purchased from Vitrocell Embriolife (Campinas, Brazil) and Gibco (New York, USA), respectively. Cells were grown at 37°C in an atmosphere of 95% humidified air and 5% CO2. The experiments were performed in cells at logarithmic phase of growth.
4
Chitosan-Based Nanoparticle Formulation
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Chitosan (CS) of low molecular weight (LMW) (deacetylation degree, 75-85%; viscosity, 20-300 cP according to the manufacturer's data sheet), pentasodium tripolyphosphate (TPP), 2,5-diphenyl-3,-(4,5-dimethyl-2-thiazolyl) tetrazolium bromide (MTT), neutral red (NR) dye, dimethyl sulfoxide (DMSO), phosphate buffered saline (PBS) and trypsin-EDTA solution (0.5 g porcine trypsin and 0.2 g EDTA • 4Na per liter of Hanks′ Balanced Salt Solution) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Methotrexate (MTX, state purity 100.1 %) was purchased from SM Empreendimentos Farmacêuticos Ltda. (São Paulo, SP, Brazil) .
Acetonitrile was purchased from Tedia (Fairfield, USA). Fetal bovine serum (FBS) and Dulbecco's Modified Eagle's Medium (DMEM), supplemented with L-glutamine (584 mg/l) and antibiotic/antimicotic (50 mg/ml gentamicin sulphate and 2 mg/l amphotericin B), were purchased from Vitrocell (Campinas, SP, Brazil). All other reagents were of analytical grade.
Acetonitrile was purchased from Tedia (Fairfield, USA). Fetal bovine serum (FBS) and Dulbecco's Modified Eagle's Medium (DMEM), supplemented with L-glutamine (584 mg/l) and antibiotic/antimicotic (50 mg/ml gentamicin sulphate and 2 mg/l amphotericin B), were purchased from Vitrocell (Campinas, SP, Brazil). All other reagents were of analytical grade.
5
PLGA Nanoparticles with ACAT-Se Compound
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Poly(d,l -lactic-co-glycolic acid) (PLGA, 50:50, 24–38 kDa), sorbitan monooleate (Span 80®), poloxamer 407 (Pluronic® F-127), 2-2-diphenyl-1-picrylhydrazyl (DPPH), radical, 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,5-diphenyl-3-(4,5-dimethyl-2-thiazolyl) tetrazolium bromide (MTT), neutral red (NR) dye, phosphate-buffered saline (PBS), and trypsin-EDTA solution (0.5 g porcine trypsin and 0.2 g EDTA·4Na per liter of Hank’s Balanced Salt Solution) were obtained from Sigma–Aldrich (São Paulo, SP, Brazil). Fetal bovine serum (FBS) and Dulbecco’s Modified Eagle’s Medium (DMEM), supplemented with L-glutamine (584 mg/L) and antibiotic/antimycotic (50 mg/mL gentamicin sulfate and 2 mg/L amphotericin B), were purchased from Vitrocell (Campinas, SP, Brazil). All other solvents and reagents were of analytical grade.
5′-Se-(phenyl)-3-(amino)-thymidine (ACAT-Se) was obtained from the LabSelen-NanoBio (Federal University of Santa Maria, Brazil). This compound was synthesized and fully characterized as previously described (da Rosa et al., 2017).
5′-Se-(phenyl)-3-(amino)-thymidine (ACAT-Se) was obtained from the LabSelen-NanoBio (Federal University of Santa Maria, Brazil). This compound was synthesized and fully characterized as previously described (da Rosa et al., 2017).
6
Lipid Nanocarrier Formulation Components
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Sodium oleate and cholesterol were purchased from Sigma-Aldrich® (St. Louis, MO, USA). Soy phosphatidylcholine was purchased from Lucas Meyer Gmbh & Co (Hamburg, Germany). Phosphate buffer solution was prepared from sodium monohydrogen phosphate and dihydrogen phosphate that were purchased from Merck (Darmstadt, Germany). Polyoxyl-40 hydrogenated castor oil was purchased from Pharma Special (Itapevi, Sao Paulo, Brazil). Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum, gentamicin sulfate, amphotericin B, and trypsin/EDTA were purchased from Vitrocell Embriolife (Campinas, Sao Paulo, Brazil).
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