Sk mel 28 human melanoma cells

Manufactured by American Type Culture Collection

SK-MEL-28 human melanoma cells are a line of cells derived from a human melanoma. They are suitable for research and experimental purposes.

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Lab products found in correlation

B16 f1 murine melanoma cells, by American Type Culture Collection (1 mentions) Z vad fmk, by Thermo Fisher Scientific (1 mentions) B16f0 mouse melanoma cells, by American Type Culture Collection (1 mentions) Cisplatin, by Merck Group (1 mentions)

Close spelling variants of this product

SK-MEL-28 melanoma cells SK-MEL-28 cells SK-MEL-28

2 protocols using sk mel 28 human melanoma cells

Culturing Human and Murine Melanoma Cells

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SK-MEL-28 human melanoma cells and B16-F1 murine melanoma cells were obtained from ATCC. They were cultured in DMEM and 10% FBS at 37 °C in a humid atmosphere (5% CO₂, 95% air).

Lambert E., Fuselier E., Ramont L., Brassart B., Dukic S., Oudart J.B., Dupont-Deshorgue A., Sellier C., Machado C., Dauchez M., Monboisse J.C., Maquart F.X., Baud S, & Brassart-Pasco S. (2018). Conformation-dependent binding of a Tetrastatin peptide to αvβ3 integrin decreases melanoma progression through FAK/PI3K/Akt pathway inhibition. Scientific Reports, 8, 9837.

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Melanoma Cell Culture Protocols

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For in vitro studies, stock solutions of (−)-Nutlin-3 (30mM) and MLN8237 (20mM) were prepared in DMSO. The pan-caspase inhibitor Z-VAD-FMK was obtained from Molecular Probes (Eugene). Cisplatin was purchased from Sigma-Aldrich and a 10mM stock solution was prepared in DMSO. SK-Mel5, HS294T, SK-Mel28 human melanoma cells and B16F0 mouse melanoma cells were obtained from ATCC. Cells were cultured in DMEM/F12 media supplemented with 10% FBS, 100U/ml penicillin and 100ug/ml streptomycin.

Vilgelm A.E., Pawlikowski J.S., Liu Y., Hawkins O.E., Davis T.A., Smith J., Weller K.P., Horton L.W., McClain C.M., Ayers G.D., Turner D.C., Essaka D.C., Stewart C.F., Sosman J.A., Kelley M.C., Ecsedy J.A., Johnston J.N, & Richmond A. (2014). Mdm2 and Aurora A inhibitors synergize to block melanoma growth by driving apoptosis and immune clearance of tumor cells. Cancer research, 75(1), 181-193.

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