1 2 dioleoyl sn glycero 3 phosphoethanolamine n 7 nitro 2 1 3 benzoxadiazol 4 yl nbd dope

1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2–1,3-benzoxadiazol-4-yl) (NBD-DOPE), 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC), 1-palmitoyl-2-oleoyl-sn-glycero-3-phospho-L-serine (sodium salt) (POPS), and cholesterol were obtained from Avanti Polar Lipids. 5α-cholestan-3-one, cholestenone, coprostanol, and epicholesterol were obtained from Steraloids. 5(6)-carboxyfluorescein, 7-dehydrocholesterol, cholestanol, dimethyl sulfoxide (DMSO), ergosta-5,7,9(11),22-tetraen-3β-ol (DHE), hexafluoroisopropanol (HFIP), lathosterol, pregnenolone, thioflavin-T, triton X-100 and sucrose were obtained from Sigma-Aldrich. The concentrations of unlabeled lipids were determined by dry weight and that of NBD-DOPE lipids by absorbance using ε_NBD-DOPE = 21, 000 M⁻¹ cm⁻¹ at 460 nm in methanol. Sterol purity were assayed by TLC and no significant impurities were detected.

DOPC, DOPG, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-DOPE), POPC, POPS, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl) (Rho-DOPE), brain sphingomyelin, egg sphingomyelin, cholesterol were obtained from Avanti Polar Lipids. The concentrations of unlabeled lipids were determined by dry weight and that of fluorescent lipids by absorbance using εNBD-DOPE = 21, 000 M⁻¹ cm⁻¹ at 460 nm in methanol and εRho-DOPE = 95,000 M⁻¹ cm⁻¹ at 560 nm in methanol. 5(6)-carboxyfluorescein, DMSO, HFIP, thioflavin-T, triton X-100, sucrose and dextrans (FTIC-dextrans) of molecular weight molecular weight 5, 10, 70, 150 kD were obtained from Sigma-Aldrich.