Bz x700 series all in one microscope

Fresh liver tissue was immediately fixed in 10% phosphate-buffered formalin for 24 h and then processed in paraffin blocks. Four-micrometer sections were used for H&E staining. For Oil Red O (ORO) staining, fresh liver tissue was placed into a cryomold and filled with OCT Compound (Tissue-Tek), then transferred to a beaker of isopentane prechilled in liquid nitrogen. Sections were processed by HistoServ, Inc. (Germantown, MD). Unstained slides were fixed in 10% neutral buffered formalin (Sigma), rinsed in water and then transferred to 100% propylene glycol (Sigma). Slides were then stained in 0.5% ORO solution (Sigma), washed in 85% propylene glycol, then water. The slides were then counterstained with Carazzi’s hematoxylin and rinsed in water. Glycerin jelly was used to mount slides. Slide imaging was performed using a Keyence BZ-X700 series all-in-one microscope with 20× objectives, 200× magnification.

Fresh liver was placed into 10% phosphate-buffered formalin for 24 h to fix tissue and then processed into paraffin blocks. Sections were stained with hematoxylin and eosin. Histological sectioning was performed by HistoServ, Inc (Germantown, MD, USA). Slide imaging was performed using a Keyence BZ-X700 series all-in-one microscope with both 20× and 40× objectives, 200× and 400× magnification, respectively.

Immunohistochemical staining was performed on formalin-fixed paraffin embedded sections from human or mouse liver. For immunohistochemical staining, sections were deparaffinized followed by antigen retrieval with sodium citrate buffer for 2 min at 95 °C and then placed at room temperature (RT) for 30 min. Sections were blocked using 5% BSA for 30 min followed by incubation with 10% goat serum for 30 min at RT. After blocking, sections were incubated with KRT79 (Thermo-Fisher Scientific; PA5–46517) primary antibody in a humidified chamber overnight at 4 °C. Following primary antibody incubation, sections were washed and incubated with secondary antibodies for 30 min (Gene Tech Company; GK600505). Following secondary antibody incubation, the sections were washed, and DAB substrate added (Vector Laboratories, Burlingame, CA) and developed by checking for staining under a microscope. The sections were then washed and counterstained with hematoxylin for 30 s. Sections were then washed with tap water, dehydrated, and mounted. All imaging was performed using a Keyence BZ-X700 series all-in-one microscope with 20x and 40x objectives (200x magnifications) (Keyence, Osaka, Japan).