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Fetalplex animalserum complex

Manufactured by Gemini Bio
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Fetalplex™ AnimalSerum Complex is a formulation of animal-derived serum components for use in cell culture applications. It provides a standardized source of growth factors, hormones, and other critical nutrients to support the growth and proliferation of a variety of cell types.

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Lab products found in correlation

Easysep direct human neutrophil isolation kit, by STEMCELL (2 mentions) Dml28, by Caisson (2 mentions) Fetalplex animal serum complex, by Caisson (2 mentions) Percoll gradient, by GE Healthcare (2 mentions) Geneticin, by Thermo Fisher Scientific (1 mentions) Hek293t 17, by American Type Culture Collection (1 mentions) Penicillin streptomycin, by Corning (1 mentions) Streptomycin, by Corning (1 mentions) Penicillin, by Corning (1 mentions) 293t cell, by Thermo Fisher Scientific (1 mentions) Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions) Antibiotic antimycotic solution, by Thermo Fisher Scientific (1 mentions) 293 t platinum e, by Cell Biolabs (1 mentions) Hek293, by American Type Culture Collection (1 mentions) Streptomycin, by Merck Group (1 mentions) Penicillin, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Gemini Bio (1 mentions) Pc12 cells, by American Type Culture Collection (1 mentions) Fetal bovine serum (fbs), by Santa Cruz Biotechnology (1 mentions) Insulin, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

FetalPlex (27 citations)

10 protocols using fetalplex animalserum complex

1

Cell Culture and Maintenance Protocols

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HEK 293T/17 (ATCC, ATCC CRL-11268) were cultured in DMEM supplemented with 10% Fetalplex animal serum complex (Gemini Bio-Products), 2 mM L-glutamine, 100 U/ml penicillin, and 50 μg/mL streptomycin. Clonal HeLa cell lines derived from HeLa-S3 (a gift from Dr. Nyborg) expressing HBZ-Myc-His, HBZ-ΔbZIP-Myc-His, HBZ-MutZIP-Myc-His, HBZ-(LXXAA)2-Myc-His, and HBZ-ΔATG were cultured in supplemented DMEM maintained under selection with 0.5 mg/mL geneticin (Thermo Fisher Scientific)[44 (link), 68 (link)]. Jurkat and CEM cells (a gift from Dr. Nyborg), MT-2 cells (obtained from the NIH AIDS Research Program, #237) and ATL-2s cells (a gift from Dr. Matsuoka) were maintained in supplemented IMDM. TL-Om1 cells (a gift from Dr. Matsuoka) were maintained in supplemented RPMI. MT-2 cells stably expressing an shRNA that targets HBZ-SP1 (V4)(a gift from Dr. Green) were maintained under selection with 1 mg/mL geneticin [38 (link)]. MT-2 cells stably expressing an shRNA against GFP (MISSION pLKO.1-puro eGFP shRNA, Thermo Fisher Scientific SHC005), were established and maintained under selection in 1.5 μg/mL puromycin.
Rushing A.W., Rushing B., Hoang K., Sanders S.V., Péloponèse JM J.r., Polakowski N, & Lemasson I. (2019). HTLV-1 basic leucine zipper factor protects cells from oxidative stress by upregulating expression of Heme Oxygenase I. PLoS Pathogens, 15(6), e1007922.
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2

Cell Culture and Isolation Protocols

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RAW cells and HEK 293 cells were grown in Dulbecco’s Modified
Eagle Medium (supplemented with 10% Fetalplex™ Animal
Serum Complex (Gemini Bio-Products), 2 mM L-glutamine and 1%
penicillin/streptomycin). HepG2 cells were grown in DMEM with low
glucose, L-glutamine, sodium pyruvate (Caisson Labs DML28), supplemented with
10% Fetalplex™ Animal Serum Complex and 1%
penicillin/streptomycin. Cells were grown to 90% confluency, washed 1x
with phosphate buffered saline (PBS) and harvested with a cell lifter. Cell
pellets were centrifuged at 1,200 rpm, 3 min and taken up in the desired culture
medium. Whole human blood samples were received from the Stanford Blood Bank and
peripheral mononuclear cells (PMNs) were purified using the
EasySep™ Direct Human Neutrophil Isolation Kit (StemCell
Technologies).
Lentz C.S., Sheldon J.R., Crawford L.A., Cooper R., Garland M., Amieva M.R., Weerapana E., Skaar E.P, & Bogyo M. (2018). Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP). Nature chemical biology, 14(6), 609-617.
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3

Cell Culture of Cannabinoid and Opioid Receptors

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Neuro2A cells, which endogenously express cannabinoid type-1 receptors (Bromberg et al., 2008 (link)), Chinese hamster ovary (CHO-K1) cells stably expressing human cannabinoid type-2 receptors (CHO-hCB2) (Shoemaker et al., 2005 (link)) or human mu-opioid receptors (CHO-hMOR) (Seely et al., 2012 ) were used in this study. All cell lines were cultured in Dulbecco's Modification of Eagle's Medium (DMEM; Cellgro, Manassas, VA) containing 10% FetalPlex™ animal serum complex (Gemini Bio Products, West Sacramento, CA) and 1% penicillin/streptomycin (10,000 IU/ml penicillin, 10,000 μg/ml streptomycin; Cellgro, Manassas, VA). Culture media for CHO-hCB2 and CHO-hMOR cells also contained 0.5 mg/ml of the selection antibiotic geniticin (G418; Sigma-Aldrich, St. Louis, MO) to maintain stable expression of human cannabinoid type-2 receptors and human mu-opioid receptors. Cells were cultured at 37°C under 5% CO2 in a humidified incubator and harvested with PBS (10 mM)/EDTA (1 mM) when culture flasks were approximately 90–100% confluent. Cell pellets were stored at −80°C for future preparation of membrane homogenates, reseeded into flasks for continued cell line culturing or seeded into 24-well plates for adenylyl cyclase experiments (see Methods section 2.7).
Franks L.N., Ford B.M., Madadi N.R., Penthala N.R., Crooks P.A, & Prather P.L. (2014). Characterization of the intrinsic activity for a novel class of cannabinoid receptor ligands: Indole Quinuclidine analogues. European journal of pharmacology, 737, 140-148.
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4

Culturing Mouse Embryonic Fibroblasts

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293 T cells (Invitrogen), 293 T platinum E (Cell Biolabs, San Diego, CA, USA), wild-type and BRAF –/– mouse embryonic fibroblasts (MEFs) were maintained in DMEM (Gibco), 10 % FetalPlex™ animal serum complex (Gemini Bio-Products), and 1 % Antibiotic-Antimycotic Solution (Gibco). Wild-type MEF and BRAF –/– MEF were a kind gift of Dr. Catrin Pritchard (University of Leicester, Leicester, UK).
Dela Cruz F.S., Diolaiti D., Turk A.T., Rainey A.R., Ambesi-Impiombato A., Andrews S.J., Mansukhani M.M., Nagy P.L., Alvarez M.J., Califano A., Forouhar F., Modzelewski B., Mitchell C.M., Yamashiro D.J., Marks L.J., Bender J.L, & Kung A.L. (2016). A case study of an integrative genomic and experimental therapeutic approach for rare tumors: identification of vulnerabilities in a pediatric poorly differentiated carcinoma. Genome Medicine, 8, 116.
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5

Cell Culture and Isolation Protocols

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RAW cells and HEK 293 cells were grown in Dulbecco’s Modified
Eagle Medium (supplemented with 10% Fetalplex™ Animal
Serum Complex (Gemini Bio-Products), 2 mM L-glutamine and 1%
penicillin/streptomycin). HepG2 cells were grown in DMEM with low
glucose, L-glutamine, sodium pyruvate (Caisson Labs DML28), supplemented with
10% Fetalplex™ Animal Serum Complex and 1%
penicillin/streptomycin. Cells were grown to 90% confluency, washed 1x
with phosphate buffered saline (PBS) and harvested with a cell lifter. Cell
pellets were centrifuged at 1,200 rpm, 3 min and taken up in the desired culture
medium. Whole human blood samples were received from the Stanford Blood Bank and
peripheral mononuclear cells (PMNs) were purified using the
EasySep™ Direct Human Neutrophil Isolation Kit (StemCell
Technologies).
Lentz C.S., Sheldon J.R., Crawford L.A., Cooper R., Garland M., Amieva M.R., Weerapana E., Skaar E.P, & Bogyo M. (2018). Identification of a S. aureus virulence factor by activity-based protein profiling (ABPP). Nature chemical biology, 14(6), 609-617.
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6

Isolation of Lamina Propria Leukocytes

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Tissue biopsies were collected from the ileum and descending colon into Roswell Park Memorial Institute medium supplemented with 10% fetal bovine serum, streptomycin/penicillin, and L-glutamine (RP10). Lamina propria leukocytes (LPLs) were isolated using collagenase IX/DNAse digestion followed by centrifugation over a Percoll gradient (GE Healthcare, Waukesha, WI, USA) as previously described82 (link). Cells were cryopreserved in FetalPlex Animal Serum Complex (Gemini Bio-Products, West Sacramento, CA, USA) and DMSO.
Rhoades N.S., Hendrickson S.M., Prongay K., Haertel A., Gill L., Edwards R.A., Garzel L., Slifka M.K, & Messaoudi I. (2021). Growth faltering regardless of chronic diarrhea is associated with mucosal immune dysfunction and microbial dysbiosis in the gut lumen. Mucosal Immunology, 14(5), 1113-1126.
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7

Isolation of Lamina Propria Leukocytes

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Tissue biopsies were collected from the ileum and descending colon into Roswell Park Memorial Institute medium supplemented with 10% fetal bovine serum, streptomycin/penicillin, and L-glutamine (RP10). Lamina propria leukocytes (LPLs) were isolated using collagenase IX/DNAse digestion followed by centrifugation over a Percoll gradient (GE Healthcare, Waukesha, WI, USA) as previously described82 (link). Cells were cryopreserved in FetalPlex Animal Serum Complex (Gemini Bio-Products, West Sacramento, CA, USA) and DMSO.
Rhoades N.S., Hendrickson S.M., Prongay K., Haertel A., Gill L., Edwards R.A., Garzel L., Slifka M.K, & Messaoudi I. (2021). Growth faltering regardless of chronic diarrhea is associated with mucosal immune dysfunction and microbial dysbiosis in the gut lumen. Mucosal Immunology, 14(5), 1113-1126.
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8

Cell Culture Conditions for Various Cell Lines

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HGE-20 cells [50 (link)] were grown in 50:50 DMEM:F12 (DMEM-Cellgro Mediatech, Manassas, VA, USA, F12-Life Technologies, Carlsbad, CA, USA) with 10% FBS (Gemini Bio-Products, West Sacremento, CA, USA) and 100 units/ml penicillin, 0.1 mg/ml streptomycin (Sigma, St. Louis, MO, USA). HGE-20 cells were provided by Dr. Daniel Mènard, who kindly granted permission to use the cells for this work. AGS, HEK-293, A549 and MDCK cells (ATCC, Manassas, VA, USA) and HGT-1 cells [51 (link)] were grown in DMEM medium (Cellgro Mediatech) containing 4.5 g/L glucose, 2 mM L-glutamine, 8 mg/L phenol red, 100 units/ml penicillin, 0.1 mg/ml streptomycin, and 10% FBS. The growth media for A549 cells was supplemented with 20 mM HEPES. PC12 cells (ATCC) were grown in RPMI 1640 with 2 mM GlutaMAX™, 10 mM HEPES, 1 mM sodium pyruvate, 100 U/ml penicillin, and 100 μg/ml streptomycin (Sigma) supplemented with 10% FetalPlex animal serum complex (Gemini Bio-Products). Human mammary epithelial (HB2) cells [52 (link)] were maintained in DMEM supplemented with 10% FBS, 5 μg/ml hydrocortisone and 10 μg/ml insulin (Santa Cruz Biotechnology, Dallas, TX, USA). Human gastric antrum tissue was obtained through the UCLA TPCL program with a non-human subjects IRB exemption.
Marcus E.A., Tokhtaeva E., Turdikulova S., Capri J., Whitelegge J.P., Scott D.R., Sachs G., Berditchevski F, & Vagin O. (2016). Septin oligomerization regulates persistent expression of ErbB2/HER2 in gastric cancer cells. The Biochemical journal, 473(12), 1703-1718.
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9

Rhesus Macaque Splenic Leukocyte Isolation

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Spleens from rhesus macaques were collected at necropsy (euthanasia method: 8 mg/kg ketamine followed by Pentobarbital injection) in RPMI supplemented with 10% fetal bovine serum (FBS), streptomycin/penicillin, and l-glutamine. Splenic leukocytes were isolated by mechanical disruption and filtration through a 70 μm cell strainer followed by red blood cell lysis. Cells were cryopreserved in 10% DMSO in Fetalplex Animal Serum Complex (Gemini Bio-Products, Sacramento CA).
Sureshchandra S., Stull C., Ligh B.J., Nguyen S.B., Grant K.A, & Messaoudi I. (2019). Chronic heavy drinking drives distinct transcriptional and epigenetic changes in splenic macrophages. EBioMedicine, 43, 594-606.
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10

Transient and Inducible ERAD Substrate Expression

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HEK293 cells were cultured in DMEM containing 4.5 g/L glucose and L-glutamine (Mediatech), and supplemented with 10% FetalPlex animal serum complex (Gemini Bio-Products, West Sacramento, CA) at 37 °C and 5% CO2. A standard calcium-phosphate co-precipitation technique (Kingston et al., 2001 ) or X-tremeGENE HP DNA transfection reagent from Roche (06366244001) was used for transient plasmid transfections. Transient expression of ERAD substrates was monitored 48–72 hr post-transfections.
Cas9-BFP K562 human myeloma cells (a generous gift from Michael Bassik, Stanford University) stably expressing doxycycline-inducible A1ATNHK-GFP or A1ATNHK-QQQ -GFP were grown in RPMI 1640 medium (Corning) supplemented with 10% fetal bovine serum (Sigma-Aldrich), 2 mM L-glutamine (Corning), 200 μg/mL Geneticin and 4 μg/mL Blasticidin. Cells were grown in a humidified incubator at 37°C and 5% CO2. A1ATNHK-GFP or A1ATNHK-QQQ-GFP expression was induced by growing cells in complete RPMI 1640 medium supplemented with 0.1 μg/mL doxycycline (dox; Sigma-Aldrich) for 16 hr.
van der Goot A.T., Pearce M.M., Leto D.E., Shaler T.A, & Kopito R.R. (2018). Redundant and antagonistic roles of XTP3B and OS9 in decoding glycan and non-glycan degrons in ER-associated degradation. Molecular cell, 70(3), 516-530.e6.
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