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Lc3 antibody

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The LC3 antibody is a widely used tool in cell biology research. It is designed to detect the LC3 protein, which is a key component of the autophagy pathway. The LC3 antibody can be used to monitor autophagic activity in cells and tissues through various experimental techniques, such as Western blotting and immunofluorescence microscopy.

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Lab products found in correlation

Atg 5 antibody, by Novus Biologicals (2 mentions) Vectastain abc ap alkaline phosphatase, by Vector Laboratories (2 mentions) Pro par clearant, by Anatech (2 mentions) Parp p85, by Promega (1 mentions) Anti lamp1, by Developmental Studies Hybridoma Bank (1 mentions) Nrf2 antibody, by Santa Cruz Biotechnology (1 mentions) Nqo1 antibody, by Santa Cruz Biotechnology (1 mentions) β tubulin antibody, by Cell Signaling Technology (1 mentions) Nox2 antibody, by Abcam (1 mentions) Phospho α syn ser129 antibody, by Cell Signaling Technology (1 mentions) Chemidoc, by Bio-Rad (1 mentions) Ho 1 antibody, by Santa Cruz Biotechnology (1 mentions)

Close spelling variants of this product

Rabbit anti-LC3 antibody Primary antibody against microtubule-associated LC3 Anti-LC3 antibody

4 protocols using lc3 antibody

1

Immunohistochemical Analysis of Autophagy Markers

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Sections from paraffin-embedded joints were first deparaffinized in the xylene substitute Pro-Par Clearant (Anatech) and rehydrated in graded ethanol and water. Slides were cooled for 20 minutes at room temperature after antigen unmasking. After washing with PBS, sections were blocked with 5% serum for 30 minutes at room temperature. Atg5 antibody (Novus Biologicals, Littleton, CO, 1:500 dilution), LC3 antibody (MBL International, Woburn, MA, 1:1000) and PARP p85 (Promega, Madison, WI) were applied and incubated overnight at 4°C. After washing with PBS, sections were incubated with biotinylated goat anti-rabbit secondary antibody for 30 minutes at room temperature, and then incubated using Vectastain ABC-AP alkaline phosphatase (Vector Laboratories, Burlingame, CA) for 30 minutes. Specificity controls were obtained by replacing the primary antibody with non-immune rabbit immunoglobulin. Slides were washed, and sections were incubated with alkaline phosphatase substrate for 20–30 minutes.
Caramés B., Olmer M., Kiosses W.B, & Lotz M. (2015). The relationship of autophagy defects and cartilage damage during joint aging in a mouse model. Arthritis & rheumatology (Hoboken, N.J.), 67(6), 1568-1576.
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2

Quantifying Autophagy around Toxoplasma

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Endothelial cells challenged with RFP T. gondii were incubated with LC3 antibody (MBL International), or anti-LAMP-1 (Developmental Studies Hybridoma Bank) followed by secondary antibodies. Accumulation of LC3 or LAMP-1 around T. gondii was examined. At least 50 cells per well (duplicate or triplicate wells per group per experiment) were counted manually.
Corcino Y.L., Portillo J.A, & Subauste C.S. (2019). Epidermal growth factor receptor promotes cerebral and retinal invasion by Toxoplasma gondii. Scientific Reports, 9, 669.
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3

Substantia Nigra Protein Analysis

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We isolated the substantia nigra of the brain. RAPI lysate was used, the tissue was ground, and the supernatant was extracted for protein quantification. A sample with 20 μg total protein was subjected to SDS-PAGE electrophoresis. It was then transferred using a PVDF membrane and blocked with 5% skim milk. It was incubated overnight at 4° C with a mixture of primary antibodies to 0.5% skim milk TBST solution. It was then washed three times using TBST, incubated with a secondary antibody for 1 hour, and washed three times with TBST. Protein chemiluminescence was detected using ChemiDoc™ (Bio-Rad) and the ECL method.
The following primary antibodies were used: NOX2 antibody (Abcam Cat# ab129068, RRID:AB_11144496), LC3 antibody (MBL International Cat# PM036, RRID:AB_2274121), Nrf2 antibody (Santa Cruz Biotechnology Cat# sc-365949, RRID:AB_10917561), HO-1 antibody (Santa Cruz Biotechnology Cat# sc-136960, RRID:AB_2011613), NQO-1 antibody (Santa Cruz Biotechnology Cat# sc-32793, RRID:AB_628036), phospho-α-Syn Ser129 antibody (Cell Signaling Technology Cat# 23706, RRID:AB_2798868), α-Syn antibody (Cell Signaling Technology Cat# 4179, RRID:AB_1904156), and β-tubulin antibody (Cat# Cat# 1798868).
Yan J., Huang J., Liu A., Wu J., Fan H., Shen M., Lai X., Ma H., Sun W., Yang J, & Xu Y. (2020). Atorvastatin improves motor function, anxiety and depression by NOX2-mediated autophagy and oxidative stress in MPTP-lesioned mice. Aging (Albany NY), 13(1), 831-845.
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4

Immunohistochemical Analysis of Autophagy and Apoptosis Markers in Knee Joint Sections

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Knee joint serial sections (4-μm thick) were first deparaffinized in Pro-Par Clearant (Anatech) and rehydrated in a series of graded ethanol and water. Sections were blocked with 5% serum for 30 minutes at room temperature (RT) and incubated with ATG-5 antibody (1:500 dilution, Novus Biologicals. Littleton CO. NB110-53818), LC3-antibody (1:600 dilution); MBL International (Woburn, MA) PM036), and poly(ADP-ribose) polymerase (PARP) p85 (1:60 dilution, G7341, Promega, Madison, WI) overnight at 4°C. After washing with PBS, sections were incubated with biotinylated goat anti-rabbit secondary antibody for 30 minutes at RT and then with Vectastain ABC-AP alkaline phosphatase (Vector Laboratories) for 30 minutes at RT. Slides were washed and developed in alkaline phosphatase substrate for 10 to 15 minutes. Sections were dehydrated in graded ethanol and cleared in Pro-Par Clearant (Anatech), and mounted with a coverslip.
Meckes J.K., Caramés B., Olmer M., Kiosses W.B., Grogan S.P., Lotz M.K, & D’Lima D.D. (2017). Compromised autophagy precedes meniscus degeneration and cartilage damage in mice. Osteoarthritis and cartilage, 25(11), 1880-1889.
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