Pbs buffer

4-Carboxybutyltriphenylphosphonium bromide (TPP) and melatonin were purchased from Aladdin Bio-chem Technology Co. Limited (Shanghai, China). Monostearin was purchased from Shanghai Chemical Reagent Co., Ltd. (Shanghai, China). Medium-chain triglycerides was gifted from Gattefosse (Saint-Priest, France). Anti-VCAM-1 antibody was purchased from Cell Signaling Technology (Boston, United States). Fetal bovine serum (FBS), DMEM media, PBS buffer, trypsin/EDTA, and penicillin-streptomycin were purchased from Hyclone Laboratories (Logan, United States). Lipopolysaccharide (LPS), NH₂-PEG₂₀₀₀-SA and PEG₂₀₀₀-SA were purchased from Sigma-Aldrich (St Louis, MO, United States). SOD and MDA kits were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). DCFH-DA was purchased from Beyotime Biotechnology (Beijing, China).

TGN peptide and SHp peptide were purchased from ChinaPeptides (Shanghai, China). Melatonin and (4-Carboxybutyl)triphenylphosphonium bromide were purchased from Aladdin Bio-chem Technology (Shanghai, China). Maleimide-poly(ethylene glycol)2000-poly (lactic acid)5000 (Mal-PEG-PLGA) and methoxy poly (ethylene glycol)2000-poly(lactic acid)5000 (MePEG-PLGA) were purchased from Xi’an ruixi Biological Technology (Xi’an, China). JC-1 and DCFH-DA were purchased from Invitrogen (Carlsbad, USA). MTT was purchased from Solarbio technology (Beijing, China). Fetal bovine serum (FBS), DMEM media, PBS buffer, trypsin/EDTA, and penicillin–streptomycin were purchased from Hyclone Laboratories (Logan, USA). Superoxide dismutase (SOD) and Malonaldehyde (MDA) were purchased from Nanjing jiancheng Bioengineering Institute (Nanjing, China).