Adult male C57BL/6N mice of 7–10 weeks old and male ICR mice retired from breeding were purchased from Japan SLC (Shizuoka, Japan). Mice were maintained in the animal facility with constant temperature and humidity on a 12 h light/12 h dark cycle with food and water available ad libitum. Mice were first housed in groups of 4–5 mice per cage and then singly housed before behavioral experiments. All procedures for animal care and use were in accordance with the ARRIVE guidelines and the National Institutes of Health Guide for the Care and Use of Laboratory Animals and were approved by the Animal Care and Use Committees of Kobe University Graduate School of Medicine.
C57bl 6n mice
Manufactured by Japan SLC
Sourced in Japan
C57BL/6N mice are a well-established inbred mouse strain commonly used in biomedical research. They are a substrain of the C57BL/6 mouse line and are known for their stable genetic background.
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Lab products found in correlation
Icr mice, by Japan SLC (2 mentions)
Mito serum extender, by Corning (2 mentions)
C57bl 6j, by Jackson ImmunoResearch (2 mentions)
Sprague dawley rat, by Charles River Laboratories (1 mentions)
C57bl 6j mice, by Charles River Laboratories (1 mentions)
Icr mice, by Charles River Laboratories (1 mentions)
Isoflurane gas, by Fujifilm (1 mentions)
C3h hej mice, by Japan SLC (1 mentions)
Sf1 cre, by Jackson ImmunoResearch (1 mentions)
Lsm 710 laser confocal microscope, by Zeiss (1 mentions)
Apc conjugated anti ifn γ, by BioLegend (1 mentions)
Penicillin, by Nacalai Tesque (1 mentions)
Streptomycin, by Nacalai Tesque (1 mentions)
Percp cyanine5.5 conjugated anti cd4, by BioLegend (1 mentions)
Dulbecco s modified eagle s medium dmem, by Nacalai Tesque (1 mentions)
C57bl 6j, by Japan SLC (1 mentions)
Standard chow diet, by CLEA Japan (1 mentions)
Puromycin, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
Penicillin, by Fujifilm (1 mentions)
75 protocols using c57bl 6n mice
1
Adult C57BL/6N and ICR Mice Protocol
2
Aging Study in C57BL6/N Mice
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Mature male C57BL6/N mice (over 10 weeks of age) were purchased from Japan SLC Inc.
(Hamamatsu, Japan). All animal care was in accordance with institutional guidelines and was approved by the Institutional Animal Care and Use Committee of Asahikawa Medical University (No. 19102, 19156) .
(Hamamatsu, Japan). All animal care was in accordance with institutional guidelines and was approved by the Institutional Animal Care and Use Committee of Asahikawa Medical University (No. 19102, 19156) .
3
Murine Lymph Node Transfer Comparison
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All animal experiments were conducted in accordance with the Guidelines for the Care and Use of Laboratory Animals at Hokkaido University. All procedures were performed with the approval of the Institutional Animal Care and Use Committee at Hokkaido University. Fifty 8-week-old male C57BL/6N mice (Japan SLC, Inc., Hamamatsu, Japan) were used to create the murine model, and were housed in a controlled environment with food and water ad libitum. All experiments were performed under general anesthesia with isoflurane inhalation at 2.5%. Three experimental groups were created that differed only in the way the LNs were transferred: group 1, pedicled vascularized LNT (n = 20); group 2, pedicled non-vascularized LNT (n = 15); and group 3, free non-vascularized LNT (n = 15). Each transferred LN was named as follows: group 1, vascularized LN (VLN); group 2, non-VLN; and group 3, free non-vascularized LN (FLN). Sample size was determined based on similar animal studies [31, 32] , as well as our previous experience of pedicled vascularized LNT in a murine model [24] (link).
4
In utero AhR electroporation in mice
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The animal experimental protocol used in this study was approved by the Animal Care and Use Committees of Keio University and the University of Tokyo. For the in utero CA-AhR electroporation experiments, time-mated pregnant C57BL/6N mice were purchased from Japan SLC (Shizuoka, Japan). These mice were housed in an animal facility with a set temperature of 22-24°C and humidity of 40-60% under a 12-hr light/dark cycle (lights on from 08:00 to 20:00). Laboratory rodent chow (Lab MR Stock; Nosan, Kanagawa, Japan) and distilled water were provided ad libitum.
5
Generation and Culture of E.G7-OVA Cells
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E.G7-OVA cells, generated by transducing the chicken OVA gene into the murine lymphoma cell line EL4, were obtained from the American Type Culture Collection (Manassas, VA, USA). E.G7-OVA cells were grown in Roswell Park Memorial Institute (RPMI) 1640 (Sigma Aldrich, St. Louis, MO, USA) supplemented with 50 μM of β-mercaptoethanol, 10% fetal calf serum, 10 mM 2-[4-(2-Hydroxyethyl)-1-piperazinyl]ethanesulfonic acid (HEPES), 1 mM sodium pyruvate and 100 units/mL of penicillin/streptomycin. Cells were cultured at 37 °C in a 5% CO2 incubator.
C57BL/6N mice (female, 6 weeks of age) were purchased from Japan SLC (Shizuoka, Japan).
C57BL/6N mice (female, 6 weeks of age) were purchased from Japan SLC (Shizuoka, Japan).
6
Adult Male C57BL/6N Mice Experiments
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All experiments complied with the applicable guidelines and regulations. The animal care and use procedure followed protocols that have been approved by the Animal Research Committee of Kansai Medical University. All experiments complied with the ARRIVE guidelines (https://arriveguidelines.org/arrive-guidelines/experimental-animals).
Nine adult male C57BL/6N mice (Japan SLC, Inc., Shizuoka, Japan) aged 12-18 weeks were used in the experiments. The mice were housed under a standard 12-h light/dark cycle and were given access to food and water ad libitum.
Nine adult male C57BL/6N mice (Japan SLC, Inc., Shizuoka, Japan) aged 12-18 weeks were used in the experiments. The mice were housed under a standard 12-h light/dark cycle and were given access to food and water ad libitum.
7
Mice Housing and Care Protocol
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All experiments used 8-week-old male adult C57BL/6N mice (body weight 20-25 g; Japan SLC, Hamamatsu, Japan). The animals were housed in controlled temperatures under a 12-h light/dark cycle (lights on from 07:00-19:00), with food and water ad libitum. All experiments were carried out in accordance with the Guide for Animal Experimentation of Chiba University. The experimental procedure was approved by the Animal Care and Use Committee of Chiba University (permission number: 25-270).
8
Aged C57BL/6N Mouse Tissue Collection
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C57BL/6 N mice (4-months and 3-days old) were obtained from Japan SLC Co. (Shizuoka, Japan) and housed in rooms maintained at constant temperature and humidity with a 12 h light/dark cycle. For this study, tissues were obtained from mice that had to be killed owing to excessive breeding. This study was approved by the Animal Care and Use Committee of RIKEN and Okayama University and performed in accordance with the Japanese Council on Animal Care guidelines (No. AH15–09-5, No. OKU-2012259). All animal procedures were performed in accordance with approved protocols.
9
Dietary Intervention Study in Mice
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The animal experiment protocol was drafted according to the guidelines of the Animal Care and Use Committee of Kagoshima University (Permission NO. A12005). Male C57BL/6N mice (5 weeks old) from Japan SLC Inc. (Shizuoka, Japan) were housed separately in cages with wood shaving bedding, under controlled light (12 h light/day) and temperature (23.5 °C), and free access to water and feed. After acclimatizating for one week, the mice were randomly divided into the following five groups (n = 5): normal diet (ND) group, Western diet (WD) group, WD + 0.5% PC group, WD + 0.5% PG group, and WD + 0.25% PC + 0.25% PG (CG) group (Figure 1 and Table A1 ). ND contained 3% lard and 3% soybean oil; WD contained 30% lard, 3% soybean oil, and 1.5% cholesterol. Normal water was provided to ND group, and 4% fructose water was provided to WD groups. Mice were sacrificed at 18 weeks of age after overnight fasting.
10
Murine Model of Pathogen Exposure
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All experiments were performed in accordance with the Regulations and Guidelines on Scientific and Ethical Care and Use of Laboratory Animals of the Science Council of Japan, enforced on June 1, 2006, and approved by the Institutional Animal Care and Use Committee at Niigata University (permit number 39). Six-week-old male C57BL/6N mice were obtained from Japan SLC, Inc. (Shizuoka, Japan). The mice were acclimatized under specific pathogen-free conditions and fed regular chow and sterile water until the commencement of infection at 8 weeks of age.
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