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7 protocols using sodium chloride (nacl)

1

Synthesis and Characterization of Gold Nanoparticles

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Hydrogen tetrachloroaurate trihydrate (HAuCl4·3H2O), folic acid (FA), 11-mercaptoundecanoic acid (MUA) and trichloroacetic acid were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). Bovine serum albumin (BSA, fraction V) and amino acids (Ile, Pro, Arg, Gly, Gln, Ser, Ala, Leu, His) were purchased from Beijing Dingguo Changsheng Biotech Co. (Beijing, China). Other routine reagents like HCl, NaOH, NaCl, KCl, MgCl2, CaCl2, Na2CO3, NaHCO3, NaH2PO4, Na2HPO4 and Na3PO4 were purchased from Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). All chemicals were of analytical reagent grade and were used without further purification. The water used throughout all the experiments was purified through a Millipore system.
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2

Synthesis and Characterization of Iron Citrate Complexes

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All chemicals were commercially available and analytical grade unless otherwise stated. FeC6H5O7·5H2O (99.5% purity) and NaCl (99.5% purity) were obtained from Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). High-purity argon (Ar, 99.999% purity), high-purity hydrogen (H2, 99.999% purity), and acetylene (C2H2, 99.9% purity) were purchased from Tianjin Liufang Industrial Gas Co., Ltd. (Tianjin, China). MB (C16H18ClN3S·3H2O, 98.5% purity), an organic dye, was purchased from Tianjin Benchmark Chemical Reagent Co., Ltd. (Tianjin, China).
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3

Heavy Water-based NMR Protocol

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The main reagents included heavy water (Cambridge Isotope Laboratories, Inc.,); NaCl, K2HPO4, and NaH2PO4 (Tianjin Guangfu Fine Chemical Research Institute); an NMR tube, and distilled water.
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4

Sensitive Detection of Tetracycline Antibiotics

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All chemicals were of analytical reagent grade and were used as received.
Distilled water (18.2 MΩ cm -1 ) was used throughout the current study. Oxytetracycline (OTC), tetracycline (TC), hydrogen tetrachloroaurate(III) tetrahydrate (HAuCl4• 4H2O), and streptavidin (SA) were purchased from Heowns Biochem Technology Co., Ltd. (Tianjin, China). Doxycycline (DC) was purchased from Meryer Chemical Technology Co., Ltd. (Shanghai, China). Hydroxylammonium chloride (NH2OH• HCl) was purchased from Macklin Biochemical Co., Ltd. (Shanghai, China). NaOH, K2HPO4, and metal salts including NaCl, KCl, MgCl2, CuCl2, MnCl2, FeCl3, and CaCl2 were purchased from Tianjin Guangfu Fine Chemical Research Institute (Tianjin, China). Phenylalanine (Phe), histidine (His), arginine (Arg), valine (Val), and isoleucine (Ile) were purchased from Yuanye Bio-Technology Co., Ltd. (Shanghai, China). Bovine serum albumin (BSA) and calmodulin (CaM) were purchased from Sigma-Aldrich (Beijing, China). Other reagents were obtained from Sinopharm Chemical Reagent Co., Ltd. (Beijing, China).
A stock solution of HAuCl4 (1%, w/v) was prepared by dissolving 1 g HAuCl4 in 100 mL of ultrapure water and stored at 4°C. The preparation of 0.1 M of Mcllvaine-EDTA buffer (pH 4) was carried out by dissolving 5.9 g trisodium citrate dihydrate, 13.8 g Na2HPO4•12H2O, and 18.6 g Na2EDTA•12H2O in 500 mL of ultrapure water.
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5

Salecan Extraction and Characterization

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Salecan (Mw~2000 kDa, 95% purity) was obtained from Sichuan Synlight Biotech Ltd. (Chengdu, China). NaCl (analytical grade) was purchased from Tianjin Guangfu Technology Development Co., Ltd. (Tianjin, China).
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6

Degradation of 2,4-D via Activated Persulfate

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2,4-D (Beijing, Lideshi chemical technology Co., Ltd, > 97%) was used as the target organic pollutant. Potassium persulfate (>99.5%) and NaCl (>99.8%) were purchased from Tianjin Guangfu technology development Co., Ltd. Sulfuric acid (H 2 SO 4 ), sodium hydroxide (NaOH) (96%) and methanol (MeOH) (99.9%) were purchased from Tianjin Kemiou Chemical Reagent Co., Ltd. Phosphoric acid (85%) was obtained from Tianjin Wind Boat chemical reagents Technology Co., Ltd. Tert-butyl alcohol (TBA) (>99.5%) was purchased from the Sinopharm Group Chemical Reagent Co., Ltd. (Shanghai, China). NaNO 3 , Na 2 CO 3 (>99.8%) and FeSO 4 (>99%) were purchased from Tianjin Yongda chemical reagents development center. 5,5-Dimethyl -1-pyridine N-oxide (DMPO) (Shanghai Aladdi biological technology Co., Ltd) was used to scavenge SO 4
À and OH to test electron paramagnetic resonance (EPR).
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7

Synthesis of Co3O4@C@PGC Nanosheets

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All the chemicals were of analytical grade and were used without further purification. Firstly, 1.8 g of glucose (Sinopharm Chemical Reagent Co., LTD. AR), 0.582 g of Co(NO3)2.6H2O(Sinopharm Chemical Reagent Co., LTD. AR) and 15 g of NaCl (Tianjin Guangfu Technology Development Co., LTD. AR)were dissolved in 50 ml of deionized water under magnetic stirring until a transparent blue solution was obtained. Then, the solution was transfered into a drying oven and dried at 80 oC for 24 h. The dry block of crystal was ground to fine powder in an agatemortar. The composite powder was heated in a tubular vacuum furnace at 750 oC for 2 h under flowing N2. Finally, it was annealed again at 300 oC for 4 h under air. Once cooled to room temperature, the as-synthesized black powder was washed by deionized water several times to remove NaCl, and then Co3O4@C@PGC nanosheets were obtained. For comparison, Co3O4/C composites were also synthesized by carbonizing the mixture of Co(NO3)2·6H2O and glucose without adding NaCl at the same conditions. Meanwhile, Co3O4 NPs were also synthesized by sintering Co3O4@C@PGC nanosheets at 600 oC for 2 h in air. And PGC nanosheets were obtained by immersing Co3O4@C@PGC nanosheets in plenty of nitric acid for 4 h at 70 oC with magnetic stirring to remove the Co3O4 NPs.
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