Our primary breast cancer samples were extracted with RNeasy Mini Kit (Qiagen), followed by quality control with RNA 6000 chips (Agilent Bioanalyzer). RNA species with RIN values > 7 were sent to the Genomics Core of Yale Stem Cell Centre. Ribo-depleted RNA-Seq was conducted with 100 bp read length. The read count quantification and FPKM data were generated as above. The RNA-Seq reads of our samples will be deposited to GEO upon publishing of this manuscript.
Rna 6000 chips
Manufactured by Agilent Technologies
The RNA 6000 chips are laboratory equipment designed for the analysis and quantification of RNA samples. These chips utilize advanced microfluidic technology to perform automated electrophoretic separation and detection of RNA molecules within a small sample volume. The core function of the RNA 6000 chips is to provide a standardized and efficient method for the assessment of RNA integrity and concentration, which is crucial for various downstream applications in molecular biology and genomics research.
Automatically generated - may contain errors
2 protocols using rna 6000 chips
1
Breast Cancer RNA-Seq Profiling
2
Total RNA Extraction from Blood Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total RNA was extracted from the blood samples using the Tempus Spin RNA isolation kit (Applied Biosystems) according to manufacturer’s instructions. RNA yields (13–160 μg/sample) were measured using the NanoDrop Spectrophotometer (NanoDrop Technologies). The integrity of the RNA was tested with capillary gel-electrophoresis using RNA 6000 Chips (Agilent Technologies) analyzed on the Agilent 2100 Bioanalyzer. RIN values ranged from 7 to 9.6. Samples were stored at −80°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!