Sections were simultaneously incubated with a mixture of the primary antibodies against IL-4 (diluted 1:20; Abnova Co., Taipei, Taiwan) and pro-form of major basic protein 1 (proMBP1), followed by their respective secondary antibodies such as donkey anti-rabbit IgG conjugated with Cy3TM (red signal; Jackson ImmunoResearch Laboratories, West Grove, PA, USA) and donkey anti-mouse IgG conjugated with Alexa Fluor488 (green signal; Molecular Probes, Eugene, OR, USA) (n = 6). Double-stained sections were observed with a Nikon ECLIPSE TS100 fluorescence microscope. A yellowish signal due to merging of red and green signals was considered to be the result of colocalization of IL-4 in basophils (identified by ProMBP1, a basophil cell marker).
Interleukin 4 (il 4)
Manufactured by Abnova
Sourced in United States
IL-4 is a recombinant protein that functions as a cytokine. It is involved in the regulation of immune responses and inflammation.
Automatically generated - may contain errors
Lab products found in correlation
Donkey anti mouse igg, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488, by Thermo Fisher Scientific (1 mentions)
Eclipse ts100 fluorescence microscope, by Nikon (1 mentions)
Telomerase pcr elisa kit, by Roche (1 mentions)
Tnf α, by Abnova (1 mentions)
Interleukin 6 (il 6), by Abnova (1 mentions)
Il 10, by Abnova (1 mentions)
2 protocols using interleukin 4 (il 4)
1
Basophil Identification via Dual Immunostaining
2
Cytokine Profiling and Telomerase Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to examine the cytokines secreted by cells, enzyme-linked immunosorbent assay (ELISA) was performed to detect Flt3lig, B cell activating factor (BAFF), IL-2, IL-6, IL-10, LIF, SCF, IL-22, TNF-α, IL-15, IL-4 and IL-7 (Abnova Corporation, Abnova, USA) in the culture supernatant harvested after 72 hours of cultivation without FBS. The quantification of telomerase activity of cells was assayed using the telomerase PCR ELISA kit (Roche Diagnostics GmbH, Mannheim, Germany) based on the telomeric repeat amplification protocol (TRAP).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!