The MCF-7 breast cancer cell line was obtained from the Type Culture Collection of the Chinese Academy of Sciences (Shanghai, China). MCF-7 cells were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum, 100 U/ml penicillin and 100 µg/ml streptomycin (complete medium; Invitrogen; Thermo Fisher Scientific, Inc., Waltham, MA, USA) at 37°C in 5% CO2. HSA and PEI were purchased from Sigma-Aldrich; Merck Millipore (Darmstadt, Germany). The enhanced green fluorescent protein (EGFP)-N1 plasmid (pEGFP-N1) was a gift from Professor Xinhan Zhao of Xi'an Jiaotong University (Xi'an, China). The study was approved by the Ethics Committe of Xi'an Jiaotong University.
Mcf 7 breast cancer cell line
Manufactured by National Collection of Authenticated Cell Cultures
Sourced in China
The MCF-7 breast cancer cell line is a widely used cell culture model derived from a human breast adenocarcinoma. It is a common tool for cancer research and drug development studies.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by Thermo Fisher Scientific (1 mentions)
Roswell park memorial institute (rpmi), by Thermo Fisher Scientific (1 mentions)
Human serum albumin (hsa), by Merck Group (1 mentions)
Egf af 100 15, by Thermo Fisher Scientific (1 mentions)
4 methylumbelliferone 4 mu, by Merck Group (1 mentions)
Tgf β1, by Thermo Fisher Scientific (1 mentions)
Epidermal growth factor (egf), by Thermo Fisher Scientific (1 mentions)
2 protocols using mcf 7 breast cancer cell line
1
MCF-7 Cell Culture Protocol
2
Cell Culture and Cytokine Treatment
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The MCF-7 breast cancer cell line and the A549 lung adenocarcinoma cell line were obtained from the Type Culture Collection of the Chinese Academy of Sciences, Shanghai, China. The cells were cultured in RPMI-1640 medium supplemented with 10% fetal calf serum (HyClone, Logan, UT, USA) and 100 U/ml of penicillin and 100 mg/l of streptomycin at 37°C in a 5% CO2 humidified atmosphere. Logarithmic phase cells were used in the experiments. In some cases, 10% fetal calf serum was changed to serum-free medium depending on the experiment. The cells were treated with EGF at 10 ng/ml or TGF-β1 at 5 ng/ml for 24 h. EGF (AF-100-15) and TGF-β1 (AF-100-18B) were purchased from PeproTech (Rocky Hill, NJ, USA). 4-Methylumbelliferone (4-MU) was obtained from Sigma-Aldrich (St. Louis, MO, USA).
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