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Theophyline

Manufactured by Merck Group
Sourced in India

Theophyline is a laboratory instrument used for the detection and quantification of theophylline, a methylxanthine drug commonly used to treat respiratory conditions such as asthma and chronic obstructive pulmonary disease (COPD). The instrument utilizes analytical techniques to measure the concentration of theophylline in biological samples, enabling healthcare professionals to monitor and adjust medication dosages effectively.

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2 protocols using theophyline

1

Preparation of Phytochemical Stock Solutions

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Stock solutions of phytochemicals were prepared as 10 mg ml–1 concentration and stored at 4oC for further use. Myrtenol, α-bisabolol, carvacrol, phytic acid and ethyl linoleate were procured from Sigma-Aldrich, India. Cineole, theophyline, borneol, oleic acid and α-pinene were procured from Alfa Aeser, India. MEthanol (Sigma-Aldrich, India) was used to dissolve Myrtenol, ethyl linoleate and theophyline. Ethanol (Sigma-Aldrich, India) was used to dissolve α-bisabolol, phytic acid, Cineole, theophyline, borneol, oleic acid and α-pinene.
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2

Caffeine and Methylxanthine Extraction Protocol

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Caffeine, theophyline and theobromine were from Sigma-Aldrich (Darmstadt, Germany). Methanol for HPLC was from Scharlab (Barcelona, Spain). Ultrapure Milli-Q water (Millipore Iberica, Barcelona, Spain) with a resistivity of 18.2 MΩ·cm (at 25 °C) was used. Glacial acetic acid and petroleum ether, both for analysis, were supplied by PanReac AppliChem (Barcelona, Spain). Two hundred mg ExtraBond C18 solid-phase extraction (SPE) cartridges were from Scharlab.
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