Malondialdehyde mda kit

Human serum albumin solution was purchased from Baxter (Vienna, Austria). Vitamin C (AA) injection was supplied by Shenya Animal Healthcare (Shanghai, China). Mouse monoclonal antibodies to heme oxygenase 1 (HO-1, #MA1-112) and mouse monoclonal antibodies to L-ferritin (#MA5-14733) were purchased from Thermo Fisher Scientific (Rockford, United States). Rabbit monoclonal antibody to ß-Actin (#AC026) was purchased from Abclonal (Wuhan, China). Rabbit monoclonal antibody to nuclear factor erythroid 2-related factor 2 (Nrf2, # ab92946), rabbit monoclonal antibodies 4-hydroxy-2-nonenal (4-HNE, #ab46545), goat anti-mouse IgG H&L (HRP) (#ab6789), goat anti-rabbit IgG H&L (HRP) (#ab6721), a catalase (CAT) activity assay kit (#ab83464), a glutathione peroxidase (GPx) assay kit (#ab102530), and an 8-hydroxy 2 deoxyguanosine (8-OHdG) assay kit (#ab201734) were purchased from Abcam (Cambridge, MA, United States). A total antioxidant capacity (T-AOC) assay kit (#A015-2-1) and superoxide dismutase (SOD) kit (#A001-3-1) were obtained from Jiancheng Biotech. (Nanjing, China). A malondialdehyde (MDA) kit (#S0131S) was supplied by Beyotime Biotechnology (Shanghai, China). An enhanced chemiluminescence (ECL) kit (#PF001) was a product from Affinity Biosciences (Changzhou, China).

The lipopolysaccharide (LPS) and Glutathione (GSH) assay kits were purchased from Sigma-Aldrich Chemical Company (St. Louis, USA). DAPI was from Thermo Fisher Scientific. Mouse recombinant MFG-E8 protein was from R&D Systems (Minneapolis, USA). F12-Dulbecco's modified Eagle's medium (DMEM), fetal bovine serum (FBS), phosphate-buffered saline (PBS), and penicillin/streptomycin came from Life Technologies (New York, USA). The Iba-1 antibody was from Wako (Fuji, Japan). The TRIzol reagent was purchased from Invitrogen (Waltham, USA). Kelch-like ECH-associated protein 1 (Keap-1), Nuclear factor E2-related factor 2 (Nrf-2), Heme oxygenase 1 (HO-1), and GAPDH antibodies were purchased from Cell Signaling Technology (Beverly, USA). The Malondialdehyde (MDA) kit, Superoxide Dismutase (SOD) kit, ROS assay kit, TNF-α ELISA kit, and IL-6 ELISA kit were purchased from Beyotime (Shanghai, China).