Human IEC: the small intestine enterocyte cell line CCL-241 and the colonocyte cell line CCL-248, human lymphoblastoid T-cell line CEM, and human monoblastoid tumor cell line U937 were purchased from ATCC (Manassas, VA) and grown in the respective ATCC complete growth media at 37°C in a humid, 5% CO2 incubator. To differentiate into macrophages, the U937 cells were treated with 200 nM PMA (Sigma-Aldrich Corporation, St. Louis, MO) and allowed to adhere to tissue culture plate for 3 days [49 (link)]. The full length recombinant (r)SLURP-1 and rSLURP-2 were manufactured at Virusys Corporation (Sykesville, MD), as detailed elsewhere [50 ]. The previously characterized anti-SLURP-1 and -2 monoclonal antibodies 336H12-1A3 and 341F10-1F12, respectively [46 (link), 47 (link)], were from Research and Diagnostic Antibodies (North Las Vegas, NV). Normal mouse IgG (NIgG) was obtained from Santa Cruz Biotechnology, Inc. (Santa Cruz, CA). Primary mouse antibodies to human ICAM, IL-1β, IL-6, IL-10, TNFα, and IFNγ receptor (IFNγR) and ELISA kits for measuring human IL-6 and CXCL10 were purchased from R&D Systems (Minneapolis, MN). The IL-8 ELISA kit was from BD Biosciences (San Jose, CA). Both recombinant IL-1β and INFγ were from R&D Systems and both E. coli DNA and LPS from E. coli K12 strain (LPS-EK) were purchased from InvivoGen (San Diego, CA).
Lps from e coli k12 strain lps ek
Manufactured by InvivoGen
Sourced in United States
LPS from E. coli K12 strain (LPS-EK) is a laboratory product that serves as a source of lipopolysaccharide (LPS) derived from the Escherichia coli K12 strain. LPS is a major component of the outer membrane of Gram-negative bacteria and is commonly used in research applications.
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Lab products found in correlation
Recombinant il 1β, by R&D Systems (1 mentions)
Il 8 elisa kit, by BD (1 mentions)
Ccl 241, by American Type Culture Collection (1 mentions)
Inf γ, by R&D Systems (1 mentions)
Phorbol myristate acetate (pma), by Merck Group (1 mentions)
Tnf α, by R&D Systems (1 mentions)
Elisa kit, by R&D Systems (1 mentions)
Pam3cks4, by InvivoGen (1 mentions)
Pam2cks4, by InvivoGen (1 mentions)
2 protocols using lps from e coli k12 strain lps ek
1
Differentiation and Characterization of Immune Cells
2
Heterologous expression of Streptococcal pilus
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Bacterial strains are listed in Table 1 . L. lactis MG1363 was grown under static conditions at 28°C, in M17 broth supplemented with 5% glucose and 200 µg mL–1 kanamycin where appropriate. The plasmid containing the complete pilus operon from the genome of S. pyogenes serotype M1/T1 strain SF370 (ATCC 700294) under the control of the lactococcal promoter P2323 was introduced into L. lactis by electroporation as described previously.23 Escherichia coli BL21 was grown at 37°C in LB containing 50 µg mL–1 ampicillin and 30 µg mL–1 chloramphenicol. The TLR2 agonist Pam3CKS4, the TLR2/6 agonist Pam2CKS4 and the TLR4 agonist LPS from E. coli K12 strain (LPS‐EK) were obtained from InvivoGen (San Diego, CA, USA). ToxOut Endotoxin‐Free Water from BioVision (San Francisco, CA, USA) was utilized as a negative control and diluent in the cell‐based assays.
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