Anti mouse lgg hrp linked antibody

Manufactured by Cell Signaling Technology

Sourced in United States

The Anti-mouse lgG HRP-linked antibody is a secondary antibody conjugated with horseradish peroxidase (HRP). It is designed to detect and quantify mouse immunoglobulin G (IgG) in various immunoassays and immunoblotting applications.

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Anti-mouse HRP-linked antibody Anti-mouse HRP-linked HRP-linked antibody HRP anti-mouse

3 protocols using anti mouse lgg hrp linked antibody

Antibody-based Protein Expression Analysis

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The following antibodies were all purchased from Cell Signaling Technology (CST, MA, USA): anti-JNK, anti-phospho-JNK, anti-c-JUN, anti-phospho-c-JUN, anti-Cyclin-D1, anti-AKT2, anti-phospho-AKT2, anti-cleaved PARP89, anti-mouse lgG HRP-linked antibody, anti-rabbit lgG HRP-linked antibody. β-actin, mouse mAb was purchased from YCASEN (YCASEN, Shanghai, China). Anti-Rac1 antibody, anti-MCL1 antibody and anti-PARP antibody [E51] were purchased from Abcam (Cambridge, MA, USA).

Zhao J., Jie Q., Li G., Li Y., Liu B., Li H., Luo J., Qin X., Li Z, & Wei Y. (2018). Rac1 promotes the survival of H9c2 cells during serum deficiency targeting JNK/c-JUN/Cyclin-D1 and AKT2/MCL1 pathways. International Journal of Medical Sciences, 15(10), 1062-1071.

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Estrogen-Induced Oxidative Stress Response

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OVX rat injected E2 (Tocris Bioscience, Bristol, UK; catalog no. 2824), Estradiol ELISA kit (Biovision, Milpitas, CA, USA; catalog no. K7417-100). The primary antibodies used for western blot and immunofluorescence staining were 8-OHDG antibody (Abcam, Cambridge, UK; catalog no. ab48508), BiP antibody (Thermo Fisher Scientific, Lafayette, Colorado; catalog no. PA1-014A), Sigma receptor 1 antibody (Santa Cruz, California, USA; catalog no. sc-137075), p JNK antibody (Cell Signaling Technology, Boston, Massachusetts, USA; catalog no. 4668), JNK antibody (Cell Signaling Technology; catalog no. 9252), PDI antibody (Cell Signaling Technology; catalog no.3501), Ero-1α antibody (Cell Signaling Technology; catalog no.3264), Calnexin antibody (Cell Signaling Technology; catalog no.2679) and β-actin (Cell Signaling Technology; catalog no. 8457). The secondary antibodies used for western blot and immunofluorescence staining were the anti-rabbit lgG, HRP linked antibody (Cell Signaling Technology; catalog no. 7074), anti-mouse lgG, HRP linked antibody (Cell Signaling Technology; catalog no. 7076), Goat anti-Mouse IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647 (Thermo Fisher Scientific; catalog no. A32728) and Goat anti-rabbit IgG (H + L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Thermo Fisher Scientific; catalog no. A-11029).

Seo S.Y., Kang S.Y., Kwon O.S., Bang S.K., Kim S.P., Choi K.H., Moon J.Y, & Ryu Y. (2019). A mechanical acupuncture instrument mitigates the endoplasmic reticulum stress and oxidative stress of ovariectomized rats. Integrative Medicine Research, 8(3), 187-194.

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RhoA and ROCK2 Immunoblotting Protocol

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Proteins were loading to 4%-20% SurePAGE (GenScript) and immobilized onto PVDF membrane. Western analysis was conducted by blocking the membrane in TBS containing 0.1% Tween-20 and 5% Bovine Serum Albumin (Sigma #V900933-100G) for 1 hour at room temperature. Subsequently, the samples were stained overnight at 4 °C with primary antibodies: Total RhoA (CST #8789, 1:1000), Anti-ROCK2 (phospho S1366) antibody (Abcam # ab228008, 1:1000), beta actin mouse McAb (proteintech # 66009-1-Ig, 1:5000). Then membranes were washed 3X in TBST and followed by addition of secondary antibodies: Anti-Rabbit IgG HRP-Linked antibody (Cell Signaling Technology # 7074P2), Anti-mouse lgG HRP-linked antibody (Cell Signaling Technology # 7076P2). After washing three times, Supersignal Femto Western Blotting substrates (ThermoFisher Scientific) were used and bands were visualized using GEL Imaging System (GE # AI680RGB).

Fu A., Yao B., Dong T., Chen Y., Yao J., Liu Y., Li H., Bai H., Liu X., Zhang Y., Wang C., Guo Y., Li N, & Cai S. (2022). Tumor-resident intracellular microbiota promotes metastatic colonization in breast cancer. Cell, 185(8).

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