Anti irf3

Bovine serum albumin (BSA), anti-phosphotyrosine (cat no-4G10), anti-phospho-IRF3 (cat no-SAB4504031) and anti-IRF3 (cat no-SAB3500280) antibodies were from Sigma (St.Louis, MO). Neuraminidase from Arthrobacter ureafacians (10269611001) was obtained from Roche (Germany). RNeasy Mini Kit was from Qiagen (Limburg, Netherlands); Reverse Transcriptase Kit was from Promega (USA). DyNAmo Color Flash SYBR Green qPCR kit was procured from Thermo Scientific (Rockford, IL). Antibodies-Neu1 (cat no PA5-42552) and Triad3A (cat no-PA5-20079) was from Thermo Fisher Scientific (Rockford, USA). Anti-TLR4 (cat no- MTS510), anti-siglec-E (cat no-377477) antibodies and siglec-E si RNA (cat no-sc-153462) was from Santa Cruz Biotechnology. Anti-Myd88 (cat no-NBP2-27369) and anti-TRIF (cat no-NB120-13810SS) was from Novus Biologicals (Littleton, USA). The cytokine ELISA kits (IFN-β, TNF-α and IL-6) were from Elabscience and from BD-Biosciences (IFN-γ and IL-12). Neu1 plasmid DNA was obtained from Origene (MR226903). All other antibodies were from Cell signaling Technologies (Danvers, MA) unless indicated otherwise. Giemsa stain was obtained from Himedia (India).

Immunoblotting was carried out as described previously13 (link). The primary antibodies used were anti-RIG-I (IMGENEX), mAb anti-phospho IRF3 (Ser396), anti-TLR3 (Cell Signaling Technology, Beverly, MA), anti-IRF3 and anti-actin (Sigma).