Anti beta actin

To confirm drug resistance state, cells were seeded and then exposed to different concentrations of trastuzumab. After double wash with cold PBS, the monolayers were scraped into lysis buffer (Complete Lysis-M kit, Roche) and protein isolation was completed according to the instructions manual. The protein concentration was determined using Coomasie Plus Bradford Assay (ThermoScientific) and used to estimate the volume of protein yielding 15 μg, which were boiled in Laemmli buffer and resolved on an 8% SDS-PAGE and transferred onto a PVDF membrane (L-08008-001, Advansta). Primary antibodies were: anti-ErbB2/HER2 (1:1000, ab8054, Abcam) and anti-Beta-actin (1:1000, 634801, Biolegend) was served as a loading control. Mouse secondary antibody (1:10,000, 7076S; Cell Signaling) conjugated with HRP (horseradish peroxidase) was added and kept at room temperature for 60 min. The immunoblot was developed with the WesternBright Sirius Kit (K-12043-D20, Advansta).

The mouse antihuman amyloid beta1-42 (6E10), mouse antihuman tau (HT7) and mouse antihuman hyperphosphorylated tau (PHF-tau (AT8)), and anti-beta-actin (beta-actin as the loading control) were from BioLegend (San Diego, CA, USA), Thermo Fisher (Waltham, MA, USA), and Sigma-Aldrich (St. Louis, MO, USA), respectively.