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4 protocols using cobaltous chloride

1

Electrochemical Sensor Fabrication and Characterization

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Caffeic acid, protoporphyrin IX (PPIX), chitosan, N, N-dicyclohexyl carbodiimide (DCC), bicarbonate, D-glucose, uric acid, L-ascorbic acid, acetaminophen, ferric chloride, cobaltous chloride, nickel chloride hexahydrate, cupric chloride, zinc chloride, and manganese (II) chloride tetrahydrate were purchased from Sigma-Aldrich (Saint Louis, MO, USA). N, N-dimethylformamide (DMF), and MWCNTs (30–50 nm diameter, 2 μm average length and 95% purity) were purchased from Aladdin. Acetic acid, KCl, ethanol, K3Fe[(CN)6], Na2HPO4, and citric acid were purchased from Tianjin Damao Chemical Reagent Factory (Tianjin, China). All the chemicals and reagents were of analytical-reagent grade and used as received without further purification. Deionized water was used throughout the experiments unless otherwise indicated.
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2

Chloride Compound Acquisition for Analytical Procedures

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TSC, phosphoric acid, ferric chloride (FeCl3), ferrous
chloride (FeCl2), cobaltous chloride
(CoCl2), strontium chloride (SrCl2), magnesium
chloride (MgCl2), potassium chloride (KCl), calcium chloride
(CaCl2), cesium chloride (CsCl), lithium chloride (LiCl),
mercurous chloride (HgCl), nickel chloride (NiCl2), cadmium
chloride (CdCl2), sodium chloride (NaCl), manganese chloride
(MnCl2), lead chloride (PbCl2), erbium chloride
(ErCl3), ammonium chloride (NH4Cl), copper chloride
(CuCl2), silver chloride (AgCl), bismuth chloride (BiCl3), mercuric chloride (HgCl2), fluorescein, and
cellulose dialysis tubing (2 kDa) were purchased from Sigma-Aldrich.
Hydrochloric acid (HCl) and sodium hydroxide (NaOH) were purchased
from MERCK. All chemicals were of analytical grade and used without
further purification. All solutions were prepared with Milli-Q water
(18.2 MΩ·cm).
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3

Madhuca longifolia Biosorption of Metal Ions

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Madhuca longifolia flowers were collected from our institute CSIR-CIMFR Digwadih Campus, Dhanbad. Metal ions including cobaltous chloride, lead acetate trihydrate, manganese chloride, ferric chloride, mercury chloride, chromium oxide, aluminium chloride, zinc acetate, cadmium chloride, nickel chloride, silver nitrate, and magnesium acetate were purchased from Merck India. Whatman 42 filter paper was purchased from Sigma Aldrich.
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4

VEGF-Based Protein Conjugation Protocol

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Human recombinant VEGF165, anti-VEGF165 antibody, N-hydroxysuccinimide (NHS), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), dimethylformamide (DMF) and ethanol (95%) were purchased from Sigma-Aldrich, India. Just to highlight that anti-VEGF antibody and VEGF165 were stored at 4 °C and −20 °C, respectively, before use. Freshly aqueous solutions of EDC and NHS were prepared before using. All solutions were prepared using deionized water (DI) from a Millipore system. Cobaltous chloride, potassium ferricyanide, 1,3,5-benzenetricarboxylic acid (BTC) and GO were purchased from Merck. Sodium dihydrogen orthophosphate dehydrate (Merck) and sodium phosphate dibasic dehydrate (Sigma-Aldrich) were used to prepare a 0.1 M phosphate buffer of different pH to carry out pH study. All chemicals were of analytical grade and used without any further purification.
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