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3 protocols using potassium ferrycyanide

1

Biochemical Reagent Sourcing and Preparation

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Chemical reagents such as acetylthiocholine iodide, thiobarbituric acid, sulphanilamide, reduced glutathione, DEPPD, DMSO, DPPH, trichloroacetic acid, and sodium acetate were sourced from Sigma-Aldrich (now Merck KGaA, Darmstadt, Germany). Hydrogen peroxide, methanol, acetic acid, hydrochloric acid, aluminium chloride, potassium acetate, sodium dodecyl sulphate, iron (II) sulphate, manganese chloride, potassium ferrycyanide, and ferric chloride were sourced from BDH Chemicals Ltd., (Poole, England). Except stated otherwise, all other chemicals and reagents were of analytical grades and the water was glass distilled.
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2

Comprehensive Chemical Reagents Procurement

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Chemical reagents such as acetylthiocholine iodide, sulphanilamide, reduced glutathione, n‐n‐diethyl‐para‐phenylenediamine (DEPPD), ferrous sulfate, semicarbazide were procured from Sigma‐Aldrich Co. (St Louis, Missouri, USA). Trichloroacetic acid (TCA) and sodium acetate was sourced from Sigma‐Aldrich, Chemie GmbH (Steinheim, Germany), hydrogen peroxide, methanol, acetic acid, hydrochloric acid, aluminum chloride, potassium acetate, sodium dodecyl sulfate, iron (II) sulfate, potassium ferrycyanide, and ferric chloride were sourced from BDH Chemicals Ltd. (Poole, England). Ascorbic acid and starch were products of Merck (Darmstadt, Germany). Except stated otherwise, all other chemicals and reagents were of analytical grades and the water was glass distilled.
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3

Antioxidant and Amylase Inhibitory Assays

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Chemical reagents such as Trolox (6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid), 2, 2-diphenyl-1picrylhydrazyl (DPPH), thiobarbituric acid, gallic acid, porcine pancreatic α-amylase, and 1,10-phenanthroline were procured from Sigma Aldrich Co. Trichloroacetic acid was sourced from Sigma Aldrich, Chemie GmbH (Steinheim, Germany). Hydrogen peroxide, methanol, acetic acid, hydrochloric acid, aluminium chloride, potassium acetate, sodium dodecyl sulfate, iron (II) sulfate, potassium ferrycyanide, and ferric chloride were sourced from BDH Chemicals Ltd., (Poole, England, UK). Ascorbic acid and starch were products of Merck (Darmstadt, Germany). Except stated otherwise, all other chemicals and reagents were of analytical grades and the water used was glass distilled.
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