The experimental cells of the same growth time and good condition were collected. After washing with ice‐cold PBS, cells were added to the RIPA lysis buffer for 20 minutes. After centrifuged (13 188 g) with 4°C, protein was determined using a BCA protein assay kit. Western blotting was carried out according to standard protocol. The positive stripe was analyzed by Gel Pro 4.0 optical density analysis software (Media Cybernetics, Rockville, MD, USA), and the accumulated optical density reference value of integrated optical density was measured. The following antibodies were used in this study: anti‐INHBB (ab69286; Abcam, Cambridge, UK), anti‐E‐cadherin (YT1454; ImmunoWay, Plano, TX, USA), anti‐vimentin (YT4880; ImmunoWay), anti‐VEGF‐A (YT5108; ImmunoWay), anti‐MMP‐9 (YT1892; ImmunoWay), anti‐Smad2/3 (YT4332; ImmunoWay), anti‐Smad4 (YT4337; ImmunoWay), anti‐p‐Smad2/3(T8) (YP0362; ImmunoWay), anti‐TGF‐β1 (YT4632; ImmunoWay), anti‐β‐actin (20270; ProMab, Richmond, CA, USA), anti‐p53 (10442‐1‐AP; Proteintech, Rosemont, IL, USA).
Anti vimentin
Manufactured by Immunoway
Sourced in United States
Anti-Vimentin is a laboratory reagent used to detect the presence of the vimentin protein in biological samples. Vimentin is a type III intermediate filament protein found in various cell types and is often used as a marker for certain cell types and pathological conditions. The Anti-Vimentin reagent can be used in techniques such as immunohistochemistry, Western blotting, and flow cytometry to identify and quantify vimentin expression.
Automatically generated - may contain errors
Lab products found in correlation
Anti e cadherin, by Immunoway (3 mentions)
Anti n cadherin, by Immunoway (2 mentions)
Gel pro4, by Media Cybernetics (1 mentions)
Anti vegf a, by Immunoway (1 mentions)
Anti mmp 9, by Immunoway (1 mentions)
Anti p53 10442 1 ap, by Proteintech (1 mentions)
Anti mmp 2, by Merck Group (1 mentions)
Anti gapdh, by Merck Group (1 mentions)
Anti dnmt1, by Abcam (1 mentions)
Anti cd47, by Abcam (1 mentions)
Ripa lysis buffer, by Merck Group (1 mentions)
Anti trim14, by Proteintech (1 mentions)
3 protocols using anti vimentin
1
Western Blotting Analysis of Protein Expression
2
Protein Expression Analysis by Western Blot
Check if the same lab product or an alternative is used in the 5 most similar protocols
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An equal amount of total protein was run on 12.5% SDS-PAGE, transferred to PVDF membranes (250 mA for 2 h), and probed with primary antibodies. The primary antibodies include anti-MMP2 (1:1000, 72 kDa, Sigma-Aldrich), anti-N-cadherin (1:1000, 140 kDa, ImmunoWay Biotechnology), anti-E-cadherin (1:1000, 135 kDa, ImmunoWay Biotechnology), anti-Vimentin (1:1000, 57 kDa, ImmunoWay Biotechnology), anti-ADAM9 (1:1000, 72 kDa, Affinity Biosciences), anti-Snail (1:1000, 29 kDa, ImmunoWay Biotechnology), anti-DNMT1 (1:1000, 183 kDa, Abcam), anti-CD47 (1:1000, 52 kDa, Abcam), and anti-GAPDH (1:1000, 37 kDa, Sigma-Aldrich). The protein bands of interest were captured after the secondary antibodies linked with peroxidase were bound to primary antibodies [26 (link)].
3
Protein expression analysis by Western blot
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Here, cells were solubilized in RIPA lysis buffer (Merck, China.). With the BCA method, we standardized the protein concentration. Lysates were resolved by 10–15% SDS polyacrylamide gels and transferred onto PVDF membranes. The membranes were incubated overnight at 4 °C with primary antibodies, including anti-TRIM14 (1:500, Proteintech, USA), anti-GAPDH (1:5000, CST, USA), and anti-E-cadherin (1:1000), anti-N-cadherin (1:1000), anti-Vimentin (1:1000) (Immunoway, USA), followed by another incubation with appropriate secondary antibodies (the anti-rabbit or anti-mouse antibodies were from Immunoway) at 25 °C 2 h. The blots were developed using ECL.
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