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Infinite m1000 pro multimode plate reader

Manufactured by Tecan
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The Infinite M1000 Pro Multimode Plate Reader is a laboratory instrument designed for high-performance absorbance, fluorescence, and luminescence measurements. It is capable of performing a wide range of assays in microplates, cuvettes, and other sample formats.

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Lab products found in correlation

Alt activity assay kit, by Merck Group (1 mentions) Rat mouse insulin elisa kit, by Merck Group (1 mentions) Ethanol assay kit, by Merck Group (1 mentions) Biomol green reagent, by Enzo Life Sciences (1 mentions) Glycogen assay kit, by Cayman Chemical (1 mentions) Triglyceride quantification kit, by Merck Group (1 mentions)

Close spelling variants of this product

Infinite® M1000 PRO multimode reader Infinite M1000 multimode plate reader Infinite M1000 Pro plate reader Infinite M1000 Pro reader Infinite M1000 Pro plate M1000 multimode plate reader Infinite M1000 plate reader M1000 Pro plate reader Infinite M1000 Pro Infinite M1000 reader

6 protocols using infinite m1000 pro multimode plate reader

1

Serum Biomarkers in Ethanol Study

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The baseline blood samples were taken 2 weeks prior to starting the control or ethanol diet. Serum was collected from the terminal blood of the mice at sacrifice. For the analysis of serum acetate at the 2-week time point of the ethanol diet, 20 μl of hind-limb venous blood was collected into a capillary. The isolated serum together with the baseline and sacrifice serum samples was analyzed with the acetate colorimetric assay kit (MAK086, Sigma-Aldrich). The ALT activity assay kit (MAK052, Sigma-Aldrich) was used to determine serum ALT levels. Serum total cholesterol, HDL cholesterol and triglyceride levels were determined by an enzymatic method (Roche Diagnostics), and LDL + VLDL cholesterol values were calculated using the Friedewald equation [16 (link)]. Serum insulin levels were determined with the Rat/Mouse Insulin ELISA kit (EZRMI-13 K, Millipore). Blood glucose concentrations were measured with a glucometer and the homeostatic model assessment-insulin resistance (HOMA-IR) index was calculated from the blood glucose and serum insulin values. Blood ethanol levels were determined with the Ethanol Assay Kit (Sigma Aldrich, MAK076-1KT) by a coupled enzyme reaction that results in a colorimetric product. The absorbances of the colorimetric products were determined with the Infinite M1000 Pro Multimode Plate Reader (Tecan).
Laitakari A., Ollonen T., Kietzmann T., Walkinshaw G., Mennerich D., Izzi V., Haapasaari K.M., Myllyharju J., Serpi R., Dimova E.Y, & Koivunen P. (2019). Systemic inactivation of hypoxia-inducible factor prolyl 4-hydroxylase 2 in mice protects from alcohol-induced fatty liver disease. Redox Biology, 22, 101145.
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2

Malachite Green Assay for ATPase Activity

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ATPase activity was assessed using a malachite green assay (Biomol Green reagent, Enzo Life Sciences) as described in (Rule, et al., 2016 ). Reactions were assembled with 20 μM enzyme in 20 mM HEPES pH 8.5, 13 mM NaCl, and 1% glycerol in the presence of 5 mM ATP and 5 mM MgCl2 totaling 30 μL volume. Reactions were initiated by the addition of ATP and MgCl2 and 5 μL aliquots were removed at time 0, 15, 30, 45, and 60 min. The reactions were incubated in a water bath at 37 °C and quenched by diluting aliquots into 245 μL 1x HNG buffer followed by flash freezing in liquid nitrogen. Samples were thawed and two 50 μL aliquots from each time point were pipetted into flat bottom 96 well clear plates (Fisher). To determine amounts of orthophosphate released, 100 μL of Biomol Green reagent was added to each well, and allowed to incubate at 25 °C for 25 min. Following incubation, plates were read at 620 nm using a Tecan Infinite M1000 Pro multimode plate reader. The mean ± SEM was derived from the cumulative data generated from three separate experiments.
D’Arcy B.M., Blount J, & Prakash A. (2019). Biochemical and Structural Characterization of Two Variants of Uncertain Significance in the PMS2 Gene. Human mutation, 40(4), 458-471.
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3

Liver Glycogen and Triglyceride Quantification

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Liver glycogen content was analyzed with the Glycogen Assay Kit (Cayman Chemical, Item No. 700480) using ∼100 mg of liver and liver triglyceride content by an enzymatic method (Roche Diagnostics), the absorbances of the colorimetric products being determined with the Infinite M1000 Pro Multimode Plate Reader (Tecan).
Tapio J., Halmetoja R., Dimova E.Y., Mäki J.M., Laitala A., Walkinshaw G., Myllyharju J., Serpi R, & Koivunen P. (2022). Contribution of HIF-P4H isoenzyme inhibition to metabolism indicates major beneficial effects being conveyed by HIF-P4H-2 antagonism. The Journal of Biological Chemistry, 298(8), 102222.
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4

Assessing U-937 Cell Viability

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U-937 cell viability was assessed using a resazurin-based fluorescence
assay (46 ). For IC50determination, U-937 cells were seeded (1.5 ×104 cells/well)
in costar black 96-well clear bottom plates with LB-100 serially diluted with or
without 500 nM dexamethasone to generate the indicated final concentrations.
Following 48 hours, the resazurin solution (for details see supplemental material) was added to
reach a final concentration of 120 μM and incubated for 24 hours. At 72
hours, the relative fluorescence was measured at 540 ± 20 nm excitation
and 620 ± 20 nm emission on a Tecan Infinite M1000 Pro multimode plate
reader. All assays were conducted in quadruplicate and the mean ± SD was
derived from the cumulative data generated from four separate experiments
(n=16).
D’Arcy B.M., Swingle M.R., Papke C.M., Abney K.A., Bouska E.S., Prakash A, & Honkanen R.E. (2019). The Antitumor Drug LB-100 is a Catalytic Inhibitor of Protein Phosphatase 2A (PPP2CA) and 5 (PPP5C) Coordinating with the Active Site Catalytic Metals in PPP5C. Molecular cancer therapeutics, 18(3), 556-566.
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5

Lipid Extraction and Triglyceride Quantification

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Hepatic, placental, and gonadal WAT lipids were extracted by digestion overnight in an ethanol‐KOH solution at +55°C followed by centrifugation. The lipids in the supernatant were then precipitated on ice with MgCl2. After another centrifugation, the supernatant was assayed for triglycerides by an enzymatic method (Roche Diagnostics) and the absorbance of the colorimetric products was determined with the Infinite M1000 Pro Multimode Plate Reader (Tecan).
Sissala N., Myllymäki E., Mohr F., Halmetoja R., Kuvaja P., Dimova E.Y, & Koivunen P. (2022). Hypoxia ameliorates maternal diet‐induced insulin resistance during pregnancy while having a detrimental effect on the placenta. Physiological Reports, 10(9), e15302.
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6

Hepatic Lipid Extraction and Triglyceride Quantification

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Hepatic lipids were extracted by overnight digestion in an ethanol-KOH-solution at +55 °C followed by centrifugation at 10 000 g for 5 min. The lipids in the supernatant were precipitated on ice with MgCl2. After a second centrifugation at 10 000 g for 5 min the supernatant was assayed for triglycerides by an enzymatic method (Roche Diagnostics) and the absorbances of the colorimetric products were determined with the Infinite M1000 Pro Multimode Plate Reader (Tecan). Primary hepatocytes from WT and Hif-p4h-2gt/gt mice were treated with 0.5 mM NAC, 100 mM EtOH or combination of both for 72 h. In the latter case, cells were pretreated with 0.5 mM NAC for 1 h before the EtOH challenge. The dose of NAC and/or EtOH was added each day with a fresh medium to maintain the NAC and/or EtOH at a constant level. Triglyceride content was measured in the cell extracts of 1 × 106 viable cells using Triglyceride quantification kit (MAK266, Sigma-Aldrich) according to the manufacturer's instructions.
Laitakari A., Ollonen T., Kietzmann T., Walkinshaw G., Mennerich D., Izzi V., Haapasaari K.M., Myllyharju J., Serpi R., Dimova E.Y, & Koivunen P. (2019). Systemic inactivation of hypoxia-inducible factor prolyl 4-hydroxylase 2 in mice protects from alcohol-induced fatty liver disease. Redox Biology, 22, 101145.
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