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2 protocols using anti phospho acetyl coa carboxylaseser79

1

Western Blot Antibody Panel for AMPK

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Primary antibodies used for Western blotting: anti-phospho-AMPKα2Thr172, anti-AMPKα2, anti-phospho-acetyl-CoA carboxylaseSer79, and anti-ACC (Cell Signaling Technology, Boston, MA, USA); antiphospho-CaMKIITyr305 and anti-CaMKII (Abcam); anti-IDE, anti-GAPDH, anti-phospho-IR, IR, anti-phospho-AKT, and AKT (Santa Cruz Biotechnology).
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2

Autophagy Regulation and Signaling Pathways

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Primary antibodies were mouse monoclonal anti-ATG12 (1:100, sc-271688, Santa Cruz Biotechnology, Santa Cruz, CA), rabbit polyclonal anti-p53 (1:1000, ab131442, Abcam, Cambridge, MA), rabbit monoclonal anti-LC3 (1:1000, #3868, Cell Signaling Technology, Beverly, MA), rabbit polyclonal anti-SQSTM1/p62 (1:1000, ab91526, Abcam), rabbit monoclonal anti-ATG5 (1:1000, #12994, Cell Signaling Technology), rabbit polyclonal anti-AMPKα (1:1000, #2532, Cell Signaling Technology), rabbit polyclonal anti-phospho-AMPKα (Thr172) (1:1000, #2531, Cell Signaling Technology), rabbit polyclonal anti-phospho-Acetyl-CoA Carboxylase (Ser79) (1:1000, #3661, Cell Signaling Technology), rabbit monoclonal anti-RhoA (1:1000, #2117, Cell Signaling Technology), rabbit polyclonal anti-phospho-RhoA (Ser188) (1:500, ab125275, Abcam), rabbit polyclonal anti-phospho-MYPT1 (Thr696) (1:1000, #5163, Cell Signaling Technology), rabbit monoclonal anti-RUNX2 (1:1000, #12556, Cell Signaling Technology) and mouse monoclonal anti-Actin (1:2000, CW0096, CWBIO, Peking, China). Secondary antibodies were goat anti rabbit IgG (1:5000, 111-035-003, Jackson ImmunoResearch, West Grove, PA) and goat anti mouse IgG (1:5000, 115-035-003, Jackson ImmunoResearch). 3-MA was purchased from Sigma (St. Louis, MO). Compound C was purchased from Abcam.
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