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3h dihydroxyphenylethylamine dopamine

Manufactured by PerkinElmer
Sourced in United States

[3H]Dihydroxyphenylethylamine (dopamine) is a radiolabeled neurotransmitter used in various research applications. It is a tritium-labeled form of dopamine, which is a key neurotransmitter involved in the regulation of motor function, mood, and reward-related behavior.

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2 protocols using 3h dihydroxyphenylethylamine dopamine

1

Noribogaine and Pifithrin-μ Binding Assay

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Noribogaine was obtained from Cfm Oskar Tropitzsch GmbH (Marktredwitz, Germany). Pifithrin-μ was obtained from Sigma-Aldrich (St. Louis, MO). [3H]Dihydroxyphenylethylamine (dopamine; 36.6 Ci/mmol) and [3H]CFT (76 Ci/mmol) were obtained from Perkin Elmer (Boston, MA, USA). Cell culture media, supplements and antibiotics were purchased from Invitrogen (Carlsbad, CA, USA). Other cell culture reagents, such as bovine serum albumin (BSA) and Complete™ protease inhibitor mixture were purchased from Roche (Mannheim, Germany), SDS from BioMol GmbH (Hamburg, Germany), scintillation mixture (Rotiszint® eco plus) and Tris from Carl Roth GmbH (Karlsruhe, Germany). Anti-GFP antibody (ab290) was obtained from AbcamPlc (Cambridge, UK). Protein A-Sepharose and horseradish peroxidase linked anti-rabbit IgG1-antibody were purchased from Amersham Biosciences. All other chemicals used in experiments were of analytical grade. The 0.4% trypan blue solution was obtained from Sigma-Aldrich (St. Louis, MO).
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2

Noribogaine and Pifithrin-μ Binding Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Noribogaine was obtained from Cfm Oskar Tropitzsch GmbH (Marktredwitz, Germany). Pifithrin-μ was obtained from Sigma–Aldrich. [3H]Dihydroxyphenylethylamine (dopamine; 36.6 Ci/mmol) and [3H]CFT (76 Ci/mmol) were obtained from PerkinElmer Life Sciences. Cell culture media, supplements, and antibiotics were purchased from Invitrogen. Other cell culture reagents, such as BSA and CompleteTM protease inhibitor mixture, were purchased from Roche Applied Science, SDS from BioMol (Hamburg, Germany), scintillation mixture (Rotiszint® eco plus), and Tris from Carl Roth (Karlsruhe, Germany). Anti-GFP antibody (ab290) was obtained from Abcam (Cambridge, UK). Protein A-Sepharose and horseradish peroxidase–linked anti-rabbit IgG1 antibody were purchased from Amersham Biosciences. All other chemicals used in experiments were of analytical grade. The 0.4% trypan blue solution was obtained from Sigma–Aldrich.
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